Journal of North Khorasan

Abstract Background & Objectives: Cyprus Longus is a traditional herbal medicine widely used as a mild sedative, spasmolytic and antibacterial agent. In this study the effect of methanol extract of Cyprus Longus on visceral pain was investigated. Materials & Methods: This experimental study performed on 40 N-MRI male mice (28±3g). Animals were randomly grouped into control, positive control and receivers of Cyprus Longus extract. Control and positive control groups received normal saline and indomethacin (5mg/kg), respectively. Treatment groups were injected with 100 and 200 mg/kg of methanol extract of Cyprus Longus. All injections were performed intraperitonealy (IP). Animals were injected with acetic acid 0.6% (10ml/kg) for visceral pain induction, 30 minutes after of each intraperitoneal administration. Antinociceptive effects were recorded by counting the number of writhes during 30 minutes. The data were analyzed by SPSS statistical software and One-Way ANOVA test. The significant was shown with (p <0.05). Results: methanol extract of Cyprus Longus at 100 and 200 mg/kg and Indomethacin (5mg/kg) induced a significant reduction in pain response when compared with control group. Conclusion: This study confirms the antinociceptive properties of Cyprus Longus in comparison with Indomethacin, however other studies are necessary to confirm analgesic effect of Cyprus Longus.