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2018, 9(4): 15-19 |
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Jafarzadeh F, Raeghi S, Abad M, Vafaee F, Tabaei S J, Jafarzadeh Hesari M. Molecular and Phylogenetic Analysis of Human Hydatid Cysts. North Khorasan University of Medical Sciences 2018; 9 (4) :15-19
URL: http://journal.nkums.ac.ir/article-1-1308-en.html
URL: http://journal.nkums.ac.ir/article-1-1308-en.html
Fatemeh Jafarzadeh1 
, Saber Raeghi2 , Mohsen Abad3 , Farhad Vafaee4 , Seyed Javad Tabaei * 5, Mahsa Jafarzadeh Hesari6
, Saber Raeghi2 , Mohsen Abad3 , Farhad Vafaee4 , Seyed Javad Tabaei * 5, Mahsa Jafarzadeh Hesari6
1- MSc Student, Department of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran,Iran
2- Assistant Professor, Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
3- Assistant Professor, Department of Anesthesiology and Critical Care, Faculty of Medicine, North Khorasan,University of Medical Sciences, Bojnurd,Iran
4- Assistant Professor, Department of Surgery, Imam Ali Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Associate Professor, Department of Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran , seyyedtabaei@sbmu.ac.ir
6- MSc Student, Department of Epidemiology, Isfahan University of Medical Sciences, Isfahan, Iran
2- Assistant Professor, Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
3- Assistant Professor, Department of Anesthesiology and Critical Care, Faculty of Medicine, North Khorasan,University of Medical Sciences, Bojnurd,Iran
4- Assistant Professor, Department of Surgery, Imam Ali Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Associate Professor, Department of Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran , seyyedtabaei@sbmu.ac.ir
6- MSc Student, Department of Epidemiology, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract: (4709 Views)
Introduction: Hydatidosis is one of the most common diseases in humans and livestocks caused by the larval stage of Echinococcus granulosus. The hydatid cyst are occurred in different organs of humans as the intermediate hosts. The economic burden of the disease in Iran is very high.
Methods: The current retrospective study was conducted from 2007 to the beginning of 2015 in hospitals and health centers of Bojnourd, Iran, on 90 people who underwent surgery for hydatid cyst. In 60 subjects protoscolex was observed in the germ layer tissue of hydatid cyst studied using DNA extraction and molecular phylogenetic methods.
Results: A total of 90 patients were enrolled in the current study and followed up for 6 years. Based on the results obtained from enzymatic restriction of ITS1, RsaI, and HpaII, and COX1 sequences, all species isolated in the current study were sheep strain (G1). The COX1 gene sequences were registered in GeneBank with accession numbers KY485993 to KY486007. Phylogenetic analysis of COXI sequences showed the highest likelihood with G1 sheep strain using the maximal likelihood method.
Conclusions: Based on the demographic characteristics of the patients, the genotype of the parasite, and host-parasite relationships, the apropriate treatment and tramsmisson pattern are easy to achieve; moreover, proper prevention programs can be performes for rural sectors.
Methods: The current retrospective study was conducted from 2007 to the beginning of 2015 in hospitals and health centers of Bojnourd, Iran, on 90 people who underwent surgery for hydatid cyst. In 60 subjects protoscolex was observed in the germ layer tissue of hydatid cyst studied using DNA extraction and molecular phylogenetic methods.
Results: A total of 90 patients were enrolled in the current study and followed up for 6 years. Based on the results obtained from enzymatic restriction of ITS1, RsaI, and HpaII, and COX1 sequences, all species isolated in the current study were sheep strain (G1). The COX1 gene sequences were registered in GeneBank with accession numbers KY485993 to KY486007. Phylogenetic analysis of COXI sequences showed the highest likelihood with G1 sheep strain using the maximal likelihood method.
Conclusions: Based on the demographic characteristics of the patients, the genotype of the parasite, and host-parasite relationships, the apropriate treatment and tramsmisson pattern are easy to achieve; moreover, proper prevention programs can be performes for rural sectors.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
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