Volume 13, Issue 2 (9-2021)
2021, 13(2): 1-7 |
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Ethics code: IR.ZBMU.REC.1398.118
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Rezaee R, Tabrizian K, Anani M A, Ghorani V, Hashemzaei M. Effects of Desipramine on Morphine‑Induced Conditioned Place Preference in Mice. North Khorasan University of Medical Sciences 2021; 13 (2) :1-7
URL: http://journal.nkums.ac.ir/article-1-2351-en.html
URL: http://journal.nkums.ac.ir/article-1-2351-en.html
1- Clinical Research Development Unit, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
3- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran , hashemzaeim@zbmu.ac.ir
2- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
3- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran , hashemzaeim@zbmu.ac.ir
Abstract: (2045 Views)
Introduction: In this study, we aimed to investigate the effects of Desipramine on morphine dependence and inhibition of reinstatement using the Conditioned Place Preferences (CPP) test in mice.
Methods: In this study, two protocols were used to evaluate morphine dependency and reinstatement: In one of these protocols, 40 mice were equally assigned into 8 groups of normal saline, normal saline/morphine, three groups of morphine/DES (12.5, 25 and 50 mg/kg/day with morphine 40 mg/kg) and three groups of DES (12.5, 25 and 50 mg/kg). The time spent in the black and white compartments was calculated on day 3 and 8. In the next step, 20 mice were equally divided into 4 groups: one received normal saline/morphine and the other three groups received morphine/DES at 12.5, 25 and 50 mg/kg/d. In the other protocol, animals underwent a period of abstinence, then, they received 10 mg/kg morphine as a reminder dose in order to elucidate morphine reinforcement. Finally, the time spent in white and black box were calculated.
Results: The results confirmed animals addiction following morphine administration (40 mg/kg for four days), in CPP apparatus. In the first protocol, DES at 25 and 50 mg/kg significantly reduced morphine tendency. In the second protocol, DES at 25 and 50 mg/kg significantly reduced morphine reinstatement. But, DES 12.5 mg/kg did not commit morphine reinstatement.
Conclusions: The results confirmed that DES 25 and 50 mg/kg prohibited morphine tendency and reinstatement
Methods: In this study, two protocols were used to evaluate morphine dependency and reinstatement: In one of these protocols, 40 mice were equally assigned into 8 groups of normal saline, normal saline/morphine, three groups of morphine/DES (12.5, 25 and 50 mg/kg/day with morphine 40 mg/kg) and three groups of DES (12.5, 25 and 50 mg/kg). The time spent in the black and white compartments was calculated on day 3 and 8. In the next step, 20 mice were equally divided into 4 groups: one received normal saline/morphine and the other three groups received morphine/DES at 12.5, 25 and 50 mg/kg/d. In the other protocol, animals underwent a period of abstinence, then, they received 10 mg/kg morphine as a reminder dose in order to elucidate morphine reinforcement. Finally, the time spent in white and black box were calculated.
Results: The results confirmed animals addiction following morphine administration (40 mg/kg for four days), in CPP apparatus. In the first protocol, DES at 25 and 50 mg/kg significantly reduced morphine tendency. In the second protocol, DES at 25 and 50 mg/kg significantly reduced morphine reinstatement. But, DES 12.5 mg/kg did not commit morphine reinstatement.
Conclusions: The results confirmed that DES 25 and 50 mg/kg prohibited morphine tendency and reinstatement
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2020/10/26 | Accepted: 2021/02/16 | Published: 2021/09/14
Received: 2020/10/26 | Accepted: 2021/02/16 | Published: 2021/09/14
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