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2018, 10(1): 46-52 |
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Mehri S, Omar Rawas Y, Vahdati Hassani F, Karimi G, Hosseinzadeh H. Evaluation of the Neuroprotective Effect of Silybum Marianum Extract on Acrylamide-Induced Neurotoxicity: An In Vitro and In Vivo Study. North Khorasan University of Medical Sciences 2018; 10 (1) :46-52
URL: http://journal.nkums.ac.ir/article-1-1413-en.html
URL: http://journal.nkums.ac.ir/article-1-1413-en.html
Soghra Mehri * 
1, Yasser Omar Rawas1 , Faezeh Vahdati Hassani1 , Gholamreza Karimi1 , Hossein Hosseinzadeh1
1, Yasser Omar Rawas1 , Faezeh Vahdati Hassani1 , Gholamreza Karimi1 , Hossein Hosseinzadeh1
1- Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract: (3566 Views)
Introduction: Due to the importance of Acrylamide (ACR) neurotoxicity, investigation of the compounds, which can reduce the toxicity of ACR seems to be essential. The antioxidant effects of Silybum marianum (milk thistle) have been shown by different studies. The aim of this study was to evaluate the possible protective effects of the extract of S. marianum in prevention of the neurotoxicity of ACR in both in vitro and in vivo models.
Methods: Acrylamide toxicity in PC12 cells and the protective effect of S. marianum extracts to prevent toxicity were evaluated by the MTT test. Neurotoxicity was induced using administration of ACR (IP) for 11 days in rats. The effect of different doses of ethanolic extracts (200, 400, and 600 mg/kg) of milk thistle was evaluated in ACR-induced neurotoxicity in Wistar rats using gait score examination. In this study, vitamin E (200 mg/kg) was used as a positive control. At the end of the treatment the gait score was evaluated.
Results: The viability of the cells decreased following exposure to different concentrations of ACR for 24 hours (IC50: 5mM). The alcoholic extract of S. marianum at different concentrations after 24 hours of exposure to cells led to a significant decrease in toxicity. Furthermore, ACR at a dose of 50 mg/kg caused significant motor disorders in rats. The alcoholic extract at a dose of 600 mg/kg significantly improved animal movements as compared to the group receiving ACR.
Conclusions: The alcoholic extract of S. marianum significantly reduced the neurotoxicity of ACR in both in vitro and in vivo models, possibly through antioxidant activity.
Methods: Acrylamide toxicity in PC12 cells and the protective effect of S. marianum extracts to prevent toxicity were evaluated by the MTT test. Neurotoxicity was induced using administration of ACR (IP) for 11 days in rats. The effect of different doses of ethanolic extracts (200, 400, and 600 mg/kg) of milk thistle was evaluated in ACR-induced neurotoxicity in Wistar rats using gait score examination. In this study, vitamin E (200 mg/kg) was used as a positive control. At the end of the treatment the gait score was evaluated.
Results: The viability of the cells decreased following exposure to different concentrations of ACR for 24 hours (IC50: 5mM). The alcoholic extract of S. marianum at different concentrations after 24 hours of exposure to cells led to a significant decrease in toxicity. Furthermore, ACR at a dose of 50 mg/kg caused significant motor disorders in rats. The alcoholic extract at a dose of 600 mg/kg significantly improved animal movements as compared to the group receiving ACR.
Conclusions: The alcoholic extract of S. marianum significantly reduced the neurotoxicity of ACR in both in vitro and in vivo models, possibly through antioxidant activity.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2017/10/29 | Accepted: 2018/03/11 | Published: 2018/06/25
Received: 2017/10/29 | Accepted: 2018/03/11 | Published: 2018/06/25
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