Volume 10, Issue 1 (3-2018)
2018, 10(1): 86-91 |
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Pahang H, Nematollahi Mahani S N. Comparison of the Development of Two-cell Mouse Embryo after Co-Culture with Mouse and Human Embryonic Fibroblasts. North Khorasan University of Medical Sciences 2018; 10 (1) :86-91
URL: http://journal.nkums.ac.ir/article-1-1418-en.html
URL: http://journal.nkums.ac.ir/article-1-1418-en.html
1- Assistant Professor, Department of Anatomy, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , pahang_hasan@yahoo.com
2- Professor, Department of Anatomy, Embryology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
2- Professor, Department of Anatomy, Embryology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
Abstract: (3978 Views)
Introduction: Co-culture systems showed that feeder cells have positive effects on the development of fetus before implantation. In the present study, the effects of mouse embryonic fibroblasts (MEF) and human embryonic fibroblast (HEF) co-culture on two-cell mouse embryos in the early developmental stage were compared.
Methods: Two-cell mouse embryos extracted from the Balb/C mice uterine tubes were divided randomly to three groups as follow: the control group (G1) that cultured alone, the first experimental group (G2 group) that co-cultured with HEF, and the second experimental group (G3 group) that co-cultured with MEF. Also, the embryos in all groups cultured in the same culture medium (MEM-α (under the standard conditions such as incubation under liquid paraffin and daily evaluation for hatching stage. Then, data were analyzed by the Mann-Whitney and Student t test (P < 0.05).
Results: The results of the current study showed that the development of embryos in terms of blastulation and hatching rate was significantly increased in the G2 group than the control group (P < 0.05). Also, the blastomere in G2 group had higher quality than the other groups. It seems that fibroblasts could protect embryos against deleterious agents through oxidative stress deletion and cell metabolism regulation.
Conclusions: The current in vitro study showed that the monolayer of MEF is better than that of HEF to develop two-cell mouse embryos.
Methods: Two-cell mouse embryos extracted from the Balb/C mice uterine tubes were divided randomly to three groups as follow: the control group (G1) that cultured alone, the first experimental group (G2 group) that co-cultured with HEF, and the second experimental group (G3 group) that co-cultured with MEF. Also, the embryos in all groups cultured in the same culture medium (MEM-α (under the standard conditions such as incubation under liquid paraffin and daily evaluation for hatching stage. Then, data were analyzed by the Mann-Whitney and Student t test (P < 0.05).
Results: The results of the current study showed that the development of embryos in terms of blastulation and hatching rate was significantly increased in the G2 group than the control group (P < 0.05). Also, the blastomere in G2 group had higher quality than the other groups. It seems that fibroblasts could protect embryos against deleterious agents through oxidative stress deletion and cell metabolism regulation.
Conclusions: The current in vitro study showed that the monolayer of MEF is better than that of HEF to develop two-cell mouse embryos.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2017/10/18 | Accepted: 2018/03/11 | Published: 2018/06/26
Received: 2017/10/18 | Accepted: 2018/03/11 | Published: 2018/06/26
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