Volume 14, Issue 2 (9-2022)
2022, 14(2): 21-27 |
Back to browse issues page
Ethics code: IR.NKUMS.REC.1397.009
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Poorbarat S, Bibak B, Mohammadi A, Keshavarzi Z, Alesheikh P, Hoseinzadeh A et al . Evaluation of Anxiolytic Effects of Methanolic Extract of Lepidium draba L by Cross-Maze Method in Mice. North Khorasan University of Medical Sciences 2022; 14 (2) :21-27
URL: http://journal.nkums.ac.ir/article-1-2613-en.html
URL: http://journal.nkums.ac.ir/article-1-2613-en.html
Salar Poorbarat1 
, Bahram Bibak2 , Ameneh Mohammadi3 , Zakieh Keshavarzi2 , Peiman Alesheikh3 , Amin Hoseinzadeh1 , Farzaneh Shakeri * 4
, Bahram Bibak2 , Ameneh Mohammadi3 , Zakieh Keshavarzi2 , Peiman Alesheikh3 , Amin Hoseinzadeh1 , Farzaneh Shakeri * 4
1- Student Research Committee, North Khorasan University of Medical Sciences, Bojnurd, Iran
2- Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , f_1366_sh@yahoo.com
2- Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , f_1366_sh@yahoo.com
Abstract: (934 Views)
Introduction: Lepidium draba L (L. draba) is one of the most valuable medicinal plants belonging to the nightshade family. This study was designed to evaluate anxiolytic effects of different doses of the methanolic extract of L. draba and also the role of benzodiazepine receptors.
Method: In this study, 48 male mice weighing 25-30 g were used. The selected mice were randomly divided into 6 groups of 8. To evaluate the anxiolytic effects of methanolic extract of L. draba (50, 100 and 200 mg/kg), saline with equal volume as negative control, diazepam (2 mg/kg) as positive control and flumazenil (2 mg/kg) with high dose of extract was injected into mice to investigate the possible mechanism of the plant's anxiolytic effects, after 30 minutes they were transferred to elevated plus maze and for 5 minutes the standard indicators of anxiety assessment were observed and recorded by observing them. Instat software was used to analyze the data. The results were reported as (Mean ± SEM) and P <0.05 was considered significant.
Results: The results showed that methanolic extract of plant increased the number of entry and length of stay in the open arms in a dose-dependent manner.
Conclusion: This study shows that L. draba plant can be effective in treating anxiety probably through GABAergic system.
Method: In this study, 48 male mice weighing 25-30 g were used. The selected mice were randomly divided into 6 groups of 8. To evaluate the anxiolytic effects of methanolic extract of L. draba (50, 100 and 200 mg/kg), saline with equal volume as negative control, diazepam (2 mg/kg) as positive control and flumazenil (2 mg/kg) with high dose of extract was injected into mice to investigate the possible mechanism of the plant's anxiolytic effects, after 30 minutes they were transferred to elevated plus maze and for 5 minutes the standard indicators of anxiety assessment were observed and recorded by observing them. Instat software was used to analyze the data. The results were reported as (Mean ± SEM) and P <0.05 was considered significant.
Results: The results showed that methanolic extract of plant increased the number of entry and length of stay in the open arms in a dose-dependent manner.
Conclusion: This study shows that L. draba plant can be effective in treating anxiety probably through GABAergic system.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2021/12/12 | Accepted: 2022/05/24 | Published: 2022/09/11
Received: 2021/12/12 | Accepted: 2022/05/24 | Published: 2022/09/11
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
