Volume 1, Issue 1 (Autumn2008 2008)
2008, 1(1): 1-8 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sadeghniah H, Hosein zade H. Evaluation the effect of safranal, one of the effective compounds of Crocus sativus on oxidative injury due to global cerebral ischemia in hippocampus of rat. . North Khorasan University of Medical Sciences 2008; 1 (1) :1-8
URL: http://journal.nkums.ac.ir/article-1-423-en.html
URL: http://journal.nkums.ac.ir/article-1-423-en.html
1- , sadeghniah@mums.ac.ir
Abstract: (4460 Views)
Global cerebral ischemia in rat leads to neuronal specific damage in hippocampus and striatum. Produce free radicals of oxygen and oxidation of cell macromolecules such as unsaturated fats in cell membranes, proteins and nucleic acids are Key events in ischemia/ reperfusion and oxidative injury. In this study the effect of safranal on oxidative injury from global cerebral ischemia in hippocampus of rat was studied and hippocampus samples were studied for lipid peroxidation, amount of thiol groups (SH) and antioxidant activity. Global cerebral ischemia in four-vessel obstruction method was created in 20 minutes. For measurement of lipid peroxidation, the level of malondialdehyde in hippocampus of rat was measured by thiobarbitoric test. FRAP (ferric reducing antioxidant power) method was used for measurement of antioxidant activities of hippocampus samples. Safranal in dosages 72.75mg/kg, 145.5mg/kg, 363.75 mg/kg and 727.5 mg/kg was prescribed Intraperitoneally and normal saline (used as control, 10 ml/kg Intraperitoneally) was prescribed into animals after Induction of ischemia for 15 minutes and Prescribing of them was continued for 2 days (each 24 hours). In normal saline group significant increase was observed in amount of lipid peroxidation in comparison with sham group and significant decrease was observed in antioxidant power of hippocampus samples. Safranal in comparison with normal saline group with 727.5 mg/kg dosage reduced the MDA levels meaningful (52.31 nmol/g versus 159.7 nmol/g, p<0.001). Also significant increase was created in antioxidant power of hippocampus samples (412 µmol/g).The results of present study showed that safranal has Protective effects on oxidative stress from ischemia/ reperfusion injury.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2015/03/28 | Accepted: 2015/03/28 | Published: 2015/03/28
Received: 2015/03/28 | Accepted: 2015/03/28 | Published: 2015/03/28
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
