Volume 1, Issue 1 (Autumn2008 2008)
2008, 1(1): 47-58 |
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Tavakol Afshari J, Haj zade M, Barati M. Evaluation the effect of water extract of Alpinia galangal L. on inhibition of the HT-29 colorectal cancer cell Lines and rat fibroplast non-cancer cell line (L929).. North Khorasan University of Medical Sciences 2008; 1 (1) :47-58
URL: http://journal.nkums.ac.ir/article-1-430-en.html
URL: http://journal.nkums.ac.ir/article-1-430-en.html
1- , Jtavakol@yahoo.com
Abstract: (4188 Views)
Introduction: In new studies many anticancer compounds have been introduced from Alpinia galangal L. Anticancer effects of these compounds are from free radicals removal and inhibition of enzyme activities and cell cytotoxicity. The purpose of present study is evaluation the effect of water extract of Alpinia galangal L on HT-29 Cancer Cell Lines and rat fibroplast (L 929) in vitro.
Methods: Water extract of Alpinia galangal was placed in plates with cultured cells. morphological information (cell shape, granulocytic cell count and adhesion to the cell surface) were collected With inverted microscope for two cell lines HT-29 AND L 929 in vitro. In other part of study, cultured cells from each line were evaluated with MTT for three days and present of living cells in each of two cell lines and for each concentration of extract were calculated in Mean ± SD , and recorded. The results were evaluated with Instate and SPSS, ANOVA one-way and Tukey-Kramer or T-test software. When P<0.05 , differences are considered significant.
Results: Water extract of Alpinia galangal L in using concentrations inhibits the growth of each two line cells. The inhibitory effects of this extract on L929 cells is similar to or higher than inhibition effects on colon cancer cells; IC50 is 1500 µg/ml for healthy and cancer cell lines. In each two cell lines the percentage of Living cells will be reduced with increasing the concentration.
Conclusion: The results of this study showed that water extract of Alpinia galangal L had cytotoxic effects on cell lines HT-29 and L929. Therefor in using of water extract of Alpinia galangal L for treatment of cancer , it is better to use other kinds of Alpinia galangal L extracts that have cytotoxic effects on cancer cell lines.
Methods: Water extract of Alpinia galangal was placed in plates with cultured cells. morphological information (cell shape, granulocytic cell count and adhesion to the cell surface) were collected With inverted microscope for two cell lines HT-29 AND L 929 in vitro. In other part of study, cultured cells from each line were evaluated with MTT for three days and present of living cells in each of two cell lines and for each concentration of extract were calculated in Mean ± SD , and recorded. The results were evaluated with Instate and SPSS, ANOVA one-way and Tukey-Kramer or T-test software. When P<0.05 , differences are considered significant.
Results: Water extract of Alpinia galangal L in using concentrations inhibits the growth of each two line cells. The inhibitory effects of this extract on L929 cells is similar to or higher than inhibition effects on colon cancer cells; IC50 is 1500 µg/ml for healthy and cancer cell lines. In each two cell lines the percentage of Living cells will be reduced with increasing the concentration.
Conclusion: The results of this study showed that water extract of Alpinia galangal L had cytotoxic effects on cell lines HT-29 and L929. Therefor in using of water extract of Alpinia galangal L for treatment of cancer , it is better to use other kinds of Alpinia galangal L extracts that have cytotoxic effects on cancer cell lines.
Keywords: Alpinia galangal L, water extract, colorectal cancer, HT-29 cells, L929 cells, Cell proliferation.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2015/03/28 | Accepted: 2015/03/28 | Published: 2015/03/28
Received: 2015/03/28 | Accepted: 2015/03/28 | Published: 2015/03/28
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