[Home ] [Archive]   [ فارسی ]  
:: Volume 8, Issue 1 (7-2016) ::
JNKUMS 2016, 8(1): 59-70 Back to browse issues page
Evaluation of antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii bulbs on HeLa cell line
B Seyedalipour * 1, N Delfan-Azari , M Asadi , M Mohseni
1- , b.alipour81@gmail.com
Abstract:   (977 Views)

Background & Objectives: Natural products extracted from medicinal plants can play an important role in cancer treatment. This study was designed to investigate antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii (Baker) Boiss bulbs on HeLa cell line.

Materials & Methods: In this experimental study, ethanolic extract of Lilium ledebourii bulbs was prepared and its cytotoxicity on HeLa cell line was investigated. Hela cell line was treated with various concentrations of extract (0.156-10.0 mg ml-1) for 72 hours and growth inhibition was assayed using MTT test. In addition, antibacterial activity of the extract was examined using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Statistical analysis was performed using SPSS software.

Results: The results showed that the ethanolic extract at different concentrations significantly reduced the growth of HeLa cell compared to the control group. The most pronounced growth inhibition was %87.42 at the concentration of 1.25 mg ml-1. In addition, the results indicated that ethanolic extract of Lilium ledebourii bulbs possessed antibacterial activity. Among the aforementioned bacteria, the highest antibacterial activity was seen against S. aureus and the lowest activity against P. aeruginosa.

Conclusion: The results of this study revealed that the ethanolic extract of Lilium ledebourii bulbs possesses antibacterial activity and cytotoxicity against Hela cell line. In order to find the underlying mechanism of this activity, further research should be done.

Keywords: Lilium ledebourii, Pseudomonas aeruginosa, Staphylococcus aureus, Ethanolic extract, MTT test, HeLa cell line

Keywords: Lilium ledebourii, Pseudomonas aeruginosa, Staphylococcus aureus, Ethanolic extract, MTT test, HeLa cell line
Full-Text [PDF 351 kb]   (824 Downloads)    
Type of Study: Orginal Research | Subject: Basic Sciences
Received: 2016/06/29 | Accepted: 2016/06/29 | Published: 2016/06/29
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Seyedalipour B, Delfan-Azari N, Asadi M, Mohseni M. Evaluation of antibacterial activity and cytotoxicity of ethanolic extract of Lilium ledebourii bulbs on HeLa cell line. JNKUMS. 2016; 8 (1) :59-70
URL: http://journal.nkums.ac.ir/article-1-787-en.html


Volume 8, Issue 1 (7-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی خراسان شمالی Journal of North Khorasan University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 30 queries by YEKTAWEB 3647