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Showing 3 results for Differentiation
Evaluation of the differentiation process of human adipose tissue-derived mesenchymal stem cells to skeletal muscle-like cells: An in vitro study
Z Namjoo Germi , M Heydari , M Noruzian , R Mastari Farahani , T Altarihi , A Piryaei , V Bayati , M Heydari ,
Volume 5, Issue 1 (6-2013)
Abstract

Abstract Background Objectives: This study was carried out to evaluate the development of skeletal muscle-like cells derived from human adipose tissue mesenchymal stem cells induced by 5-azacytidine [5-aza] under in vitro condition. Materials and methods: Human adipose tissue mesenchymal stem cells [ADSCs] were purified. These cells were cultured in osteogenic or adipogenic induction medium to induce osteogenic and adipogenic differentiation. On the other hand, the skeletal myogenic differentiation potential of these cells was investigated using 3µmol 5-azacytidine [5-aza] treatment for 24 hours. Then the culture was washed out by PBS and were put in the culture medium free of 5-aza for another 4 weeks until sampling. RT-PCR assay was performed to detect the expression of specific skeletal muscle genes including Myogenin, Myh, alpha-actin, tropomyosin and myosin at 1-4 weeks after the first induction. Results: The adherent adipose tissue-derived mesenchymal stem cells exhibited a significant proliferative capacity and also showed osteogenic and adipogenic differentiation as seen in previous studies. The expression of four skeletal muscle cells genes was detected 1st to 4th week after induction in a time-dependent manner. There was a continuous increase in expression of Myogenin gene from the 1st to 4th week, whereas that of the Myh was higher after two weeks and lower after 4 weeks in comparison to the other weeks. The levels of mRNA of alpha-actin and Myosin expression were at the highest level in the fourth week. Expression of tropomyosin gene was not significant in the samples. Conclusion: The current study indicated that stimulating human adipose tissue-derived mesenchymal stem cells by 5-aza with the concentration of 3µmol under in vitro condition can lead to differentiating skeletal muscle-like cells. Furthermore prolonged culture duration may lead to more differentiated cells.


The Prediction Model of Domestic Violence Against Women Based on Alexithymia and Family Performance with Differentiation Mediation
Toktam Jafarzadeh, Ali Akbar Soleimanian, Mohammadr Mohammadpou,
Volume 12, Issue 1 (6-2020)
Abstract
Introduction: Domestic violence is the cause of most physical and psychological damages in women. The purpose of this study is to predict domestic violence against women based on Alexithymia and family performance by mediating differentiation in women visiting to comprehensive urban health services centers in Bojnourd.
Methods: The present study is a structural type and the statistical population of all women visiting in 1335 number of people. The sample size was estimated to be 250 by Cochran formula. The sampling method was a single stage cluster method. In order to measure the research variables, domestic violence, Alexithymia, family performance, and family data were analyzed using Amos software, Self-assessment mediation test method, structural equation method, path analysis.
Results: The findings showed that family performance with Alexithymia of women's differentiation is significant at a confidence level of 0.99. Conclusions: The results of this study showed that differentiation can play the mediation role in the prediction of domestic violence.

Collagen-Hydroxyapatite Scaffold Platform Incorporating Dimethyloxalylglycine Loaded Polymersomes Decorated with BMP-2 Peptide for Bone Tissue Engineering
Marzieh Mohammadi, Maryam Babaei Mahani, Mohammad Ramezani, Mona Alibolandi,
Volume 16, Issue 1 (3-2024)
Abstract
Introduction: Recent advances in the field of nanotechnology have led to the development of platforms for healing or repairing damaged tissues. The present study aimed to provide a bone extracellular matrix (ECM) mimetic platform made of hydroxyapatite and collagen loaded with nanopolymersomes encapsulating dimethyloxalylglycine (DMOG) and decorated with BMP-2 peptide in order to accelerate and increase the differentiation of mesenchymal stem cells to bone tissue.
Method: In this study, polymersome nanoparticles carrying DMOG and targeted with BMP-2 peptide were prepared. The physicochemical properties of nanoparticles were evaluated. Thereafter, it was loaded into collagen-nanohydroxyapatite scaffolds and the expression levels of osteogenic genes OCN and OPN and angiogenic genes vcam, vwf, and kdr were checked in mesenchymal stem cells.
Results: The surface charge and particle size of the synthesized nanoparticles were reported as -6 mv and 141 nm, respectively. The nanoparticles were able to slowly release 35% of the loaded DMOG within 30 days. The results demonstrated that the expression levels of OCN and OPN genes in cells exposed to polymersomes targeted with BMP-2 peptide were 27 and 2 times more than those of non-targeted polymersomes, respectively. In addition, the expression of angiogenic genes, vwf, kdr, and vcam, displayed a significant increase in targeted polymersomes compared to the non-targeted types (11.7-, 12.8-, and 2.17-fold, respectively).
Conclusion: It seems that providing an environment similar to the bone ECM by combining an osteomimetic scaffold made of collagen-nanohydroxyapatite, DMOG as an angiogenic factor, and BMP-2 as the osteogenic factor can be effective in accelerating and increasing the differentiation of mesenchymal stem cells into osteoblasts.

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