Hr Sadeghniah, H Hosein Zade,
Volume 1, Issue 1 (12-2008)
Abstract
Global cerebral ischemia in rat leads to neuronal specific damage in hippocampus and striatum. Produce free radicals of oxygen and oxidation of cell macromolecules such as unsaturated fats in cell membranes, proteins and nucleic acids are Key events in ischemia/ reperfusion and oxidative injury. In this study the effect of safranal on oxidative injury from global cerebral ischemia in hippocampus of rat was studied and hippocampus samples were studied for lipid peroxidation, amount of thiol groups (SH) and antioxidant activity. Global cerebral ischemia in four-vessel obstruction method was created in 20 minutes. For measurement of lipid peroxidation, the level of malondialdehyde in hippocampus of rat was measured by thiobarbitoric test. FRAP (ferric reducing antioxidant power) method was used for measurement of antioxidant activities of hippocampus samples. Safranal in dosages 72.75mg/kg, 145.5mg/kg, 363.75 mg/kg and 727.5 mg/kg was prescribed Intraperitoneally and normal saline (used as control, 10 ml/kg Intraperitoneally) was prescribed into animals after Induction of ischemia for 15 minutes and Prescribing of them was continued for 2 days (each 24 hours). In normal saline group significant increase was observed in amount of lipid peroxidation in comparison with sham group and significant decrease was observed in antioxidant power of hippocampus samples. Safranal in comparison with normal saline group with 727.5 mg/kg dosage reduced the MDA levels meaningful (52.31 nmol/g versus 159.7 nmol/g, p<0.001). Also significant increase was created in antioxidant power of hippocampus samples (412 µmol/g).The results of present study showed that safranal has Protective effects on oxidative stress from ischemia/ reperfusion injury.
F Soofi Zamiri , Mr Hajinezhad , A Samzadeh-Kermani , M Jahantigh , Sh Ahmadpour ,
Volume 9, Issue 2 (10-2017)
Abstract
Background & objectives:
The purpose of this study was to compare the effect of Zinc oxide nanoparticles and Zinc oxide nanocomposites on serum lipid peroxidation in rats.
Materials and Methods:Fifty-sixadult male wistar rats were divided into 8 groups (7 rats in each group: one control group and six treatment group).Rats in treatment group received intraperitoneal injections of ZnO nanoparticles (10, 20 and 40 mmol/ml) and ZnO nanocomposites (10, 20 and 40 mmol/ml) for 28 days.Control rats received distilled water for 28 days. Serum lipid peroxidatin was assayed and compared with one-way ANOVA and tukey statistical analysis. Statistical significance was set at P < 0.05.
Results:Intraperitoneal injection of ZnO nanoparticles at 20 and 40 mmol/ml significantly decreased serum lipid peroxidatin in comparisonwith the control group (P < 0.05). In contrast, intraperitoneal injection of ZnO nanocomposites had notany significant effect on lipid peroxidatin in comparisonwith the control group.
Conclusion:ZnO nanoparticles aremore effective in reduction of lipid peroxidation in comparison with the ZnO nanocomposites.
