other بررسی آثار ضدالتهابی سیتاگلیپتین در مدل التهابی ناشی از کاراژنین در پای رت علوم پایه Basic Sciences مقاله پژوهشی Orginal Research <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:nasimYW;"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA">مقدمه:</span></b> <span lang="FA">فرایندهای</span> <span lang="FA">التهابی</span> <span lang="FA">در</span> <span lang="FA">بسیاری</span> <span lang="FA">از</span> <span lang="FA">بیماری‌ها</span> <span lang="FA">نقش</span> <span lang="FA">مهمی</span> <span lang="FA">بازی</span> <span lang="FA">می‌کنند. یکی</span> <span lang="FA">از</span> <span lang="FA">شایع</span><span lang="FA">‌</span><span lang="FA">ترین</span> <span lang="FA">روش‌ها</span> <span lang="FA">برای</span> <span lang="FA">بررسی</span> <span lang="FA">التهاب،</span> <span lang="FA">مدل</span> <span lang="FA">ادم</span> <span lang="FA">پای</span> <span lang="FA">رت</span> <span lang="FA">است. سیتاگلیپتین</span> <span lang="FA">به</span><span lang="FA">‌</span><span lang="FA">عنوان</span> <span lang="FA">کنترل</span><span lang="FA">‌</span><span lang="FA">کننده</span> <span lang="FA">قند</span> <span lang="FA">خون</span> <span lang="FA">استفاده</span> <span lang="FA">می‌شود</span> <span lang="FA">و</span> <span lang="FA">می‌تواند</span> <span lang="FA">خواص</span> <span lang="FA">ضدالتهابی</span> <span lang="FA">داشته</span> <span lang="FA">باشد.</span> <span lang="FA">ازاین</span><span lang="FA">‌</span><span lang="FA">رو</span> <span lang="FA">ما</span> <span lang="FA">بر</span> <span lang="FA">آن</span> <span lang="FA">شدیم</span> <span lang="FA">که</span> <span lang="FA">این</span> <span lang="FA">فرایند</span> <span lang="FA">را</span> <span lang="FA">بررسی</span> <span lang="FA">کنیم.</span><span dir="LTR" style="font-size:9.0pt"></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="FA">روش‌ کار:</span></b><span lang="FA"> در</span> <span lang="FA">این</span> <span lang="FA">مطالعه،</span> <span lang="FA">رت‌های</span> <span lang="FA">نر</span> <span lang="FA">ویستار</span> <span lang="FA">با</span> <span lang="FA">وزن</span> <span lang="FA">180 تا 200 گرم</span> <span lang="FA">انتخاب</span> <span lang="FA">شدند. دوزهای </span><span dir="LTR">mg</span><span dir="LTR">/</span><span dir="LTR">kg</span> <span lang="FA">5، 15 و 45 از</span> <span lang="FA">سیتاگلیپتین</span> <span lang="FA">بررسی</span> <span lang="FA">شد. گروه</span> <span lang="FA">دریافت</span><span lang="FA">‌</span><span lang="FA">کننده</span> <span lang="FA">حلال</span> <span lang="FA">به</span><span lang="FA">‌</span><span lang="FA">عنوان</span> <span lang="FA">کنترل</span> <span lang="FA">منفی</span> <span lang="FA">و</span> <span lang="FA">گروه</span> <span lang="FA">کنترل</span> <span lang="FA">مثبت</span> <span lang="FA">ایندومتاسین</span> <span lang="FA">(</span><span dir="LTR">mg</span><span dir="LTR">/</span><span dir="LTR">kg</span> <span lang="FA">۵/۲) را</span> <span lang="FA">داخل</span> <span lang="FA">صفاقی</span> <span lang="FA">دریافت</span> <span lang="FA">کرد. به‌منظور</span> <span lang="FA">ایجاد</span> <span lang="FA">ادم</span> <span lang="FA">به</span> <span lang="FA">کف</span> <span lang="FA">پای</span> <span lang="FA">چپ</span> <span lang="FA">رت، 100 میکرولیتر</span> <span lang="FA">از</span> <span lang="FA">سوسپانسیون</span> <span lang="FA">کلوئیدی</span> <span lang="FA">کاراژنین</span> <span lang="FA">1 درصد به‌طور</span> <span lang="FA">موضعی</span> <span lang="FA">تزریق</span> <span lang="FA">شد. حجم</span> <span lang="FA">پا</span> <span lang="FA">با</span> <span lang="FA">کولیس</span> <span lang="FA">قبل</span> <span lang="FA">از</span> <span lang="FA">تزریق</span> <span lang="FA">کاراژنین</span> <span lang="FA">و</span> <span lang="FA">سپس</span> <span lang="FA">بعد</span> <span lang="FA">از</span> <span lang="FA">تزریق،</span> <span lang="FA">هر</span> <span lang="FA">ساعت</span> <span lang="FA">تا</span> <span lang="FA">ساعت</span> <span lang="FA">چهارم</span> <span lang="FA">اندازه‌گیری</span> <span lang="FA">شد. اعداد</span> <span lang="FA">به</span><span lang="FA">‌</span><span lang="FA">دست</span><span lang="FA">‌</span><span lang="FA">آمده</span> <span lang="FA">برحسب</span> <span lang="FA">درصد</span> <span lang="FA">افزایش</span> <span lang="FA">حجم</span> <span lang="FA">پا،</span> <span lang="FA">محاسبه</span> <span lang="FA">و</span> <span lang="FA">با</span> <span lang="FA">مقادیر</span> <span lang="FA">گروه</span> <span lang="FA">کنترل</span> <span lang="FA">مقایسه</span> <span lang="FA">شدند. </span><span lang="FA">تجزیه‌وتحلیل‌های آماری با نرم‌افزار </span><span dir="LTR">SPSS</span> <span lang="FA">نسخه 20 انجام شد.</span><span dir="LTR" style="font-size:9.0pt"></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="FA">یافته‌ها:</span></b><span lang="FA"> نتایج</span> <span lang="FA">حاصل</span> <span lang="FA">از</span> <span lang="FA">این</span> <span lang="FA">مطالعه</span> <span lang="FA">نشان</span> <span lang="FA">داد</span> <span lang="FA">که</span> <span lang="FA">گروه</span> <span lang="FA">سیتاگلیپتین</span> <span lang="FA">با</span> <span lang="FA">دوزهای </span><span dir="LTR">mg/kg</span> <span lang="FA">5 و 45</span> <span lang="FA">به</span> <span lang="FA">کاهش</span> <span lang="FA">معنی‌دار (001/0</span><span dir="LTR">P<</span><span lang="FA">)</span> <span lang="FA">در</span> <span lang="FA">میزان</span> <span lang="FA">التهاب</span> <span lang="FA">منجر شد، به</span><span lang="FA">&rlm;</span><span lang="FA">طوری ‌که</span> <span lang="FA">انفیلتراسیون</span> <span lang="FA">نوتروفیلی</span> <span lang="FA">خفیف</span> <span lang="FA">تا</span> <span lang="FA">متوسط</span> <span lang="FA">مشاهده</span> <span lang="FA">شد</span> <span lang="FA">و</span> <span lang="FA">ادم</span> <span lang="FA">خفیف</span> <span lang="FA">در</span> <span lang="FA">درم</span> <span lang="FA">و</span> <span lang="FA">نمای</span> <span lang="FA">اسفنجی</span> <span lang="FA">خفیفی</span> <span lang="FA">نیز</span> <span lang="FA">در</span> <span lang="FA">اپیدرم</span> <span lang="FA">قابل</span> <span lang="FA">مشاهده</span> <span lang="FA">بود. </span><span dir="LTR" style="font-size:9.0pt"></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="FA">نتیجه‌گیری:</span></b><span lang="AR-SA"> سیتاگلیپتین</span> <span lang="AR-SA">با</span> <span lang="AR-SA">استفاده</span> <span lang="AR-SA">از</span> <span lang="AR">مهار کننده آنزیم دی پپتیدیل پپتیداز </span><span lang="FA">با کاهش شدت التهاب </span><span lang="FA">می‌تواند</span> <span lang="FA">به</span> <span lang="FA">بهبود</span> <span lang="FA">و</span> <span lang="FA">کاهش</span> <span lang="FA">عوارض</span> <span lang="FA">بیماری‌های</span> <span lang="FA">مختلف</span> <span lang="FA">کمک</span> <span lang="FA">کند.</span><span dir="LTR" style="font-size:9.0pt"></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Arial;"><span style="line-height:107%"><b><span style="line-height:107%">Introduction</span><span style="letter-spacing:-.1pt">:</span></b> <span style="line-height:107%">Inflammatory processes play the main background role in many diseases. One of the most common methods for investigating inflammation is the <a name="_Hlk169944080">rat paw</a> edema model. Sitagliptin is used as a blood sugar controller, and it can have anti-inflammatory properties. Therefore, this study was conducted to investigate this process.<span style="font-family:Optima"></span></span></span><br> <span style="line-height:107%"><b><span style="line-height:107%">Methods</span></b><span style="line-height:107%">: In this study, rats (male Wistar weighing 180-200 grams) were selected. Doses of 5, 15, and 45 mg/kg of sitagliptin were evaluated. A group receiving solvent was considered a negative control, and another group received intraperitoneal indomethacin (2.5 mg/kg), a positive control. To induce edema, 100 microliters of carrageenan colloidal suspension (1%) were locally injected into the sole of the left <a name="_Hlk169945613">paw</a> of the rat. Paw volume was measured by calipers immediately before the carrageenan injection and then every hour after the injection until the fourth hour. The obtained measurements were calculated as the percentage increase in paw volume and compared with the values of the control group. Statistical analysis was performed using SPSS software (version 20). <span style="font-family:Optima"></span></span></span><br> <span style="line-height:107%"><b><span style="line-height:107%">Results:</span></b> <span style="line-height:107%">The results of this study showed that sitagliptin, compared to carrageenan, at doses of 5 and 45 mg/kg of rat body weight, led to a significant (P<0.001) decrease in the amount of </span><a name="_Hlk169945732"><span style="line-height:107%">edema</span></a><span style="line-height:107%"> in the paw of the animal, where mild to moderate neutrophil infiltration was observed. Slight edema in the dermis and a mild spongy appearance in the epidermis were also visible.<span style="font-family:Optima"></span></span></span><br> <b><span style="line-height:107%">Conclusions:</span></b> <span style="line-height:107%">Sitagliptin, a dipeptidyl peptidase 4 inhibitor, could reduce the intensity of inflammation potentially improving and alleviating</span> <span style="line-height:107%">the complications of various diseases</span></span></span></div> التهاب, رت, سیتاگلیپتین, کاراژنین, مهارکننده‌های دی پپتیدیل پپتیداز 4 Carrageenan, Dipeptidyl peptidase 4 inhibitors, Inflammation, Rat, Sitagliptin 43 48 http://journal.nkums.ac.ir/browse.php?a_code=A-10-25-965&slc_lang=other&sid=1 Fatemeh Ghofrani فاطمه غفرانی 100319475328460031125 0009-0009-0996-8978 No Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran دانشجوی دکترای عمومی، کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران Bahram Bibak بهرام بیباک 100319475328460031126 100319475328460031126 No Associate Professor, Department of Physiology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran دانشیار، گروه فیزیولوژی و فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران Seyede Reyhaneh Ramezanzadeh Sekeh سیده ریحانه رمضانزاده سکه 100319475328460031127 100319475328460031127 No Assistant Professor, Department of Anatomy and Pathology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran استادیار، گروه علوم تشریح و آسیب‌شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران‌ ‌ Bahareh Bahmani Mahmoud بهاره بهمنی محمود 100319475328460031128 0009-0008-8597-4619 No Department of Traditional Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran دانشجوی دکتری تخصصی، گروه داروسازی سنتی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران ‎‌ ‌ Ameneh Mohammadi آمنه محمدی 100319475328460031129 100319475328460031129 No MSc in Phytochemistry, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran کارشناس ارشد فیتوشیمی، مرکز تحقیقات فراورده‌های طبیعی و گیاهان دارویی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران Maryam Rameshrad مریم رامش راد mrameshrad@gmail.com 100319475328460031130 100319475328460031130 Yes Associate Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran- Associate Professor, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran مرکز تحقیقات فراورده‌های طبیعی وگیاهان دارویی، دانشگاه علوم پزشکی خراسان شمالی، بجنورد، ایران- دانشیار، گروه سم‌شناسی و داروشناسی، دانشکده داروسازی مشهد، دانشگاه علوم پزشکی مشهد، مشهد، ایران