Volume 11, Issue 2 (10-2019)
2019, 11(2): 59-65 |
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Alesheikh P, Feyzi P, Zarghami Moghaddam P, Mohammadi A, Kasaian J, Asad Nezhad A et al . Antibacterial Activity of Methanolic Extract of Some Native Medicinal Herbs of North Khorasan Province on Staphylococcus aureus. North Khorasan University of Medical Sciences 2019; 11 (2) :59-65
URL: http://journal.nkums.ac.ir/article-1-1824-en.html
URL: http://journal.nkums.ac.ir/article-1-1824-en.html
Peiman Alesheikh1 
, Pouyan Feyzi2 , Parastoo Zarghami Moghaddam3 , Ameneh Mohammadi4 , Jamal Kasaian5 , Asma Asad Nezhad *6 , Mahsa Taheri Bazkhane7
, Pouyan Feyzi2 , Parastoo Zarghami Moghaddam3 , Ameneh Mohammadi4 , Jamal Kasaian5 , Asma Asad Nezhad *6 , Mahsa Taheri Bazkhane7
1- Ph.D of Chinese Medicine, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
2- Ph.D of Chemical Engineering, Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran
3- Ph.D Student of Microbiology, Natural Products and Medicinal plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Ms of Phytochemistery, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Ph.D of Biotechnology, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
6- BS of Laboratory Science, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran ,asma.asadnezhad7272@gmail.com
7- BS of Laboratory Science, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
2- Ph.D of Chemical Engineering, Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran
3- Ph.D Student of Microbiology, Natural Products and Medicinal plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Ms of Phytochemistery, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Ph.D of Biotechnology, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
6- BS of Laboratory Science, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran ,
7- BS of Laboratory Science, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
Abstract: (4263 Views)
Introduction: Due to the increased antibiotic resistance of bacteria due to the uncontrolled use of antibiotics and the self-permeability of antibiotics, finding effective drugs with less complications is very important. Since traditional plants have good antibacterial properties, we decided to investigate the effect of methanolic extract of some samples from herbal plants of North Khorasan Province on Staphylococcus aureus.
Methods: Plants were collected from different parts of North Khorasan and dried in shade. After being powdered in a solvent of methanol soaked for 48 hours, the solvent was evaporated by rotary device. After drying, the extracts were dissolved in DMSO and added to the blanc discs and prepared wells. Then, the discs containing the extract were placed on the medium of the Mueller Hinton agar culture medium, cultured with Staphylococcus aureus, from DMSO as negative control and gentamicin as positive control and incubated for 24 hours and finally, the diameter of the bacterial inhibition zone Measured in millimeters.
Results: The studied plants showed antibacterial properties against gram positive bacteria of Staphylococcus aureus, among which Scutellaria pinnatifida and endocarp of walnut had the most antibacterial activity.
Conclusions: Comparison of the results showed that the diameter of the no-growth zone of Scutellaria and interstitial wood of walnut was the highest in comparison with others, which can be used for the antibacterial properties of these two herbs as an alternative to antibiotics and also natural food preservatives Made.
Methods: Plants were collected from different parts of North Khorasan and dried in shade. After being powdered in a solvent of methanol soaked for 48 hours, the solvent was evaporated by rotary device. After drying, the extracts were dissolved in DMSO and added to the blanc discs and prepared wells. Then, the discs containing the extract were placed on the medium of the Mueller Hinton agar culture medium, cultured with Staphylococcus aureus, from DMSO as negative control and gentamicin as positive control and incubated for 24 hours and finally, the diameter of the bacterial inhibition zone Measured in millimeters.
Results: The studied plants showed antibacterial properties against gram positive bacteria of Staphylococcus aureus, among which Scutellaria pinnatifida and endocarp of walnut had the most antibacterial activity.
Conclusions: Comparison of the results showed that the diameter of the no-growth zone of Scutellaria and interstitial wood of walnut was the highest in comparison with others, which can be used for the antibacterial properties of these two herbs as an alternative to antibiotics and also natural food preservatives Made.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2018/09/11 | Accepted: 2019/02/19 | Published: 2019/10/5
Received: 2018/09/11 | Accepted: 2019/02/19 | Published: 2019/10/5
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