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Showing 3 results for Promoter

Nm Samaei , M Naderi , H Khajeh , N Moradipour , N Gholipour , P *,zarghami Moghaddam ,
Volume 5, Issue 4 (3-2014)
Abstract

Abstract Background and Objectives: Most housekeeping genes, tumor-suppressor genes, and approx 40% of tissue specific genes contain G+C sequences in their promoter region that are very difficult to amplify. AEG-1 plays a significant role in invasion, metastasis, angiogenesis and chemoresistance, apoptosis, angiogenesis and aging Material and Methods:In this study, we proposed an improved polymerase chain reaction (PCR) method to be used for successful amplification of the Astrocyte elevated gene -1gene promoter region that contains >70% G+C content in a sequence of 495 bp and some transcription initiation sites in hepatocellularcarcinoma patient. Results: The results showed that using of DMSO co-amplification touchdown PCR and pfu enzyme can inhibit the formation of secondary structure in DNA Sequences and increase the PCR product. Conclusion: Therefore, this method can be recommended to amplify other GC-rich genomic templates.


Mostafa Ahangari, Shadi Hajikhani, Somayeh Hesam, Rasol Arvin, Mahsa Ghaziasgar,
Volume 10, Issue 1 (3-2018)
Abstract

Introduction: Implementing health promoting activities in a hospital results in hospital efficiency, increasing satisfaction, and life quality of patients and employees and improves the conditions of health hospitals in the competitive health market. In a hospital, different dimensions of the problem should initially be identified by extensive review of indicators and standards, global experiences, and expert views and then an appropriate program should be presented. Therefore, the present study aimed at performing a systematic review of indicators for health promotion of hospitals.
Methods: The present research was a descriptive- practical study. Sampling in this study was conducted by census due to the limited size of the study population (hospital directors of Tehran University of Medical Sciences). The data collection tool was a researcher-made questionnaire in two parts, including demographic information and factors affecting the implementation of health promoting hospitals. For data analysis, descriptive statistical analysis was performed by using SPSS version 18.
Results: For planning a health promotion project at hospitals, they should pay attention to health promotion, clinical outcomes, holistic health, quality of life, patient satisfaction, equitable and health literacy for health promotion.
Conclusions: Since the hospital responsibility goes beyond providing specialized and clinical services, all hospitals need to review their health-promoting standards, consider international experience and experts, and take appropriate actions to improve their services.

Saeedeh Ghazaey Zidanloo, Mahsa Jafarzadeh Hesari,
Volume 10, Issue 4 (3-2019)
Abstract

Introduction: X-ray diffraction studies have revealed that guanines in a DNA stands may be arranged in quartet and form a structure called G-quadruplexs. Bioinformatics studies suggested the formation of G-quadruplex structure in human crucial genes, including Wilms tumor 1 (WT1). The aim of this study was to in silico analysis of the guanine-rich sequence in the promoter region of the WT1 gene which have potential to form G-quadruplex structures.
Methods: To identify the presence of repetitive guanosine-rich sequences prone to form G-quadruplex structures in the regulatory region of the WT1 gene, the promoter sequence was extracted from the NCBI gene bank. The sequence was evaluated by the QGRS mapper software. Results: According to the data obtained from the software, and the G-scores, three appropriate sequences in the promoter region of the WT1 gene were identified with the highest probability for the formation of G-quadruplex structures. A valuable point in this study was the presence of recognition sites of large number of transcription factors in these three areas.
Conclusions: The majority of these G-quadruplex forming sequences were located in the promoter regions of the oncogenes. These discoveries have led scientists to point out that these structures may play a significant physiological role under in vivo conditions. Therefore, by identifying and targeting these sequences in the regulatory regions of the genes, it is possible to control the expression of the genes.


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