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:: Volume 9, Issue 4 (3-2018) ::
JNKUMS 2018, 9(4): 15-19 Back to browse issues page
Molecular and Phylogenetic Analysis of Human Hydatid Cysts
Fatemeh Jafarzadeh , Saber Raeghi , Mohsen Abad , Farhad Vafaee , Seyed Javad Tabaei * , Mahsa Jafarzadeh Hesari
Abstract:   (350 Views)
Introduction: Hydatidosis is one of the most common diseases in humans and livestocks
caused by the larval stage of Echinococcus granulosus. The hydatid cyst are occurred in
different organs of humans as the intermediate hosts. The economic burden of the disease
in Iran is very high.
Methods: The current retrospective study was conducted from 2007 to the beginning of
2015 in hospitals and health centers of Bojnourd, Iran, on 90 people who underwent
surgery for hydatid cyst. In 60 subjects protoscolex was observed in the germ layer tissue
of hydatid cyst studied using DNA extraction and molecular phylogenetic methods.
Results: A total of 90 patients were enrolled in the current study and followed up for 6
years. Based on the results obtained from enzymatic restriction of ITS1, RsaI, and HpaII,
and COX1 sequences, all species isolated in the current study were sheep strain (G1).
The COX1 gene sequences were registered in GeneBank with accession numbers
KY485993 to KY486007. Phylogenetic analysis of COXI sequences showed the highest
likelihood with G1 sheep strain using the maximal likelihood method.
Conclusions: Based on the demographic characteristics of the patients, the genotype of
the parasite, and host-parasite relationships, the apropriate treatment and tramsmisson
pattern are easy to achieve; moreover, proper prevention programs can be performes for
rural sectors.
Keywords: Hydatid Cyst Phylogenetic Bojnurd Epidemiology
Full-Text [PDF 699 kb]   (145 Downloads)    
Type of Study: Orginal Research | Subject: Basic Sciences
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
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Jafarzadeh F, Raeghi S, Abad M, Vafaee F, Tabaei S J, Jafarzadeh Hesari M. Molecular and Phylogenetic Analysis of Human Hydatid Cysts. JNKUMS. 2018; 9 (4) :15-19
URL: http://journal.nkums.ac.ir/article-1-1308-en.html


Volume 9, Issue 4 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی خراسان شمالی Journal of North Khorasan University of Medical Sciences
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