Abstract Background and Objectives: Trichomonas vaginalisis a protozoan pathogen of the human urogenital tract. It infects usually vagina and urethra in women but may infect the bartholin or scan glands, cervix and bladder. In infected men, the organism is often seen in the distal urethra but rarely, involves prostate gland, seminal vesicle and epididymal.This study was designed to provide a data base on investigation of the overall prevalence and clinical findings and compare of diagnostic methods for detection of Trichomoniasis . Materials and Methods: In this study (a cross sectional) total of 400 vaginal samples were examined with wet smear and pap smear and diamond culture medium for Trichomoniasis in Chabahar in 2012. The confirmed clinical findings by gynecologist recorded in patient information forms. The data analysis was done using the SPSS software version 18.0 and frequency tables generated. The chi-square and T student tests was used to determine significant relationship between categorical variables at a significant P value of less than or equal to 0.05. Results: In this study prevalence of Trichomoniasis were reported 39 (9.75%) for diamond culture media and 27 (6.75%) for pap smear and 25(6.25%) for wet mounts direct microscopy slides, respectively. However , there was no statistically significant difference between the clinical symptoms seen in patients examined with infection (P>0.05). There was statistically significant difference between patient reported sings with infection (P<0.05). Conclusion: considering high prevalence of Trichomonas vaginalis , asymptomatic infected careers and excessive treatment before the exact laboratory diagnosis , it is recommended to perform reliable testing methods routinely in addition to wet mounts ,culture methods
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