Journal of North Khorasan

Abstract Background & Objectives: Since the incidence rate of colon carcinoma is growing significantly and natural products and medicinal plants are widely used in cancer treatment, the cytotoxic effects of two unstudied medicinal plants have been studied. Material & Methods: Different concentration of the hexane, chloroform and ethanol fractions of Uvaria grandiflora Roxb. (leaves and stem barks) and Diospyros wallichii King&Gamble.(fruits and stem barks) were subjected to cytotoxic investigation against HTC-116 cell line by using MTT technique. In this study, quercetin was used as a positive control. Results: The chloroform fraction of Uvaria grandiflora Roxb. stem barks and the chloroform fraction of Diospyros wallichii King&Gamble. Fruits exhibit the highest growth inhibiting activities among all the tested samples with the GI50 value of 3.85 µg/ml and 4.58 µg/ml, respectively. Conclusion: From the obtained results, it was concluded that the Naphthoquinones derivatives from Diospyros wallichii and Flavonoids from Uvaria grandiflora are responsible for pharmacological and biological activities. Therefore, more investigations and isolations are recommended