Volume 14, Issue 4 (3-2023)
2023, 14(4): 63-74 |
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Bedrood Z, Masjedi E, Vahdati Hassani F, Ghasemzadeh Rahbardar M, Hosseinzadeh H, Abnous K et al . Evaluation the Effect of Crocin on Bisphenol A-Induced Memory Impairment in Rats: Role of ERK, CaMKII, and CREB Proteins in Hippocampus. North Khorasan University of Medical Sciences 2023; 14 (4) :63-74
URL: http://journal.nkums.ac.ir/article-1-2698-en.html
URL: http://journal.nkums.ac.ir/article-1-2698-en.html
Zeinab Bedrood1 
, Elaheh Masjedi1 , Faezeh Vahdati Hassani2 , Mahboobeh Ghasemzadeh Rahbardar3 , Hossein Hosseinzadeh4 , Khalil Abnous5 , Soghra Mehri *6
, Elaheh Masjedi1 , Faezeh Vahdati Hassani2 , Mahboobeh Ghasemzadeh Rahbardar3 , Hossein Hosseinzadeh4 , Khalil Abnous5 , Soghra Mehri *6
1- MSc in Toxicology, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2- Ph.D. in Toxicology, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3- Ph.D. in Physiology, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4- Professor, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5- Professor, Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
6- Associate Professor, Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2- Ph.D. in Toxicology, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3- Ph.D. in Physiology, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
4- Professor, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5- Professor, Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
6- Associate Professor, Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Abstract: (1056 Views)
Introduction: Bisphenol A (BPA) is a toxic chemical that is used as a monomer in the production of plastic products. The BPA has an endocrine-disrupting effect and induces damage to the nervous system. Crocin, an active ingredient of Crocus sativus L. has neuroprotective effects. Therefore, this study aimed to evaluate crocin’s effects on BPA-induced memory impairment in rats and the levels of Extracellular Signal-Regulated Kinases (ERK), Ca2+/Calmodulin-Dependent Protein Kinase-II (CaMKII), and Cyclic AMP-Response Element-Binding Protein (CREB) proteins in the hippocampus.
Method: Bisphenol A (100 mg/kg, gavage) and crocin (10, 20, and 40 mg/kg, IP) were administered to adult male rats for 28 days. Memory and learning functions were evaluated by the Morris water maze, and the level of proteins was determined using the Western blot method.
Results: Administration of the BPA (100 mg/kg) significantly induced memory impairment and reduced the levels of ERK, p.ERK, CaMKII, and p.CREB proteins in the BPA group, compared to the control group. On the other hand, crocin (20 mg/kg) markedly improved learning and memory impairment and increased the levels of ERK, p.ERK, and CaMKII proteins in the hippocampus, compared to the BPA (100 mg/kg) group.
Conclusion: Administration of crocin (20 mg/kg) improves memory and learning defects induced by BPA (100 mg/kg). The enhancement of the p.ERK, ERK, and p. CaMKII protein levels in the hippocampus has an important role in crocin protective effects
Method: Bisphenol A (100 mg/kg, gavage) and crocin (10, 20, and 40 mg/kg, IP) were administered to adult male rats for 28 days. Memory and learning functions were evaluated by the Morris water maze, and the level of proteins was determined using the Western blot method.
Results: Administration of the BPA (100 mg/kg) significantly induced memory impairment and reduced the levels of ERK, p.ERK, CaMKII, and p.CREB proteins in the BPA group, compared to the control group. On the other hand, crocin (20 mg/kg) markedly improved learning and memory impairment and increased the levels of ERK, p.ERK, and CaMKII proteins in the hippocampus, compared to the BPA (100 mg/kg) group.
Conclusion: Administration of crocin (20 mg/kg) improves memory and learning defects induced by BPA (100 mg/kg). The enhancement of the p.ERK, ERK, and p. CaMKII protein levels in the hippocampus has an important role in crocin protective effects
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2022/08/24 | Accepted: 2022/12/17 | Published: 2023/02/26
Received: 2022/08/24 | Accepted: 2022/12/17 | Published: 2023/02/26
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