Volume 15, Issue 1 (Spring 2023)
2023, 15(1): 10-17 |
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Ethics code: IR.NKUMS.REC.1400.076
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Salahi E, Asgharzadeh A, Amani A, Besharati M, Tatari M. Design of Nanoemulsions Containing Essential Oil of Artemisia Kopetdaghensis: Investigation of Antioxidant Properties and Antimicrobial Activity on Two Isolates from Clinical Specimens. North Khorasan University of Medical Sciences 2023; 15 (1) :10-17
URL: http://journal.nkums.ac.ir/article-1-2741-en.html
URL: http://journal.nkums.ac.ir/article-1-2741-en.html
1- PhD Candidate in Horticultural Science, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2- Assistant Professor, Department of Horticulture, Shirvan Branch, Islamic Azad University, Shirvan, Iran ,asg.ahmad@yahoo.com
3- Professor of Pharmaceutics, Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Postdoc Researcher, Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant Professor, Department of Agronomy, Shirvan Branch, Islamic Azad University, Shirvan, Iran
2- Assistant Professor, Department of Horticulture, Shirvan Branch, Islamic Azad University, Shirvan, Iran ,
3- Professor of Pharmaceutics, Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Postdoc Researcher, Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant Professor, Department of Agronomy, Shirvan Branch, Islamic Azad University, Shirvan, Iran
Abstract: (975 Views)
Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.
Method: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.
Results: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.
Conclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil
Method: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.
Results: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.
Conclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2022/07/13 | Accepted: 2022/12/17 | Published: 2023/05/27
Received: 2022/07/13 | Accepted: 2022/12/17 | Published: 2023/05/27
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