Journal of North Khorasan

Abstract Background and Objectives: mainly due to increased unintentional side-effects of synthetic antioxidants and antimicrobials, This study evaluated the antioxidant and some antibacterial properties of different aerial parts’ extracts in Cyperus longus. Material and Methods: The Hexane, Dichloromethan and Methanolic aerial parts’ extracts of Cyperus longus were obtained by maceration method. Antibacterial activity of extracts determined by Disc Diffusion Method. The antioxidant capacity of extracts was assessed through DPPH method. The results of DPPH assay were showed by IC50. The total phenolic content was measured using the Folin-Ciocalteu’s reagent. The total flavonoid content was assessed by a colorimetric method based on Aluminum Chloride, has been associated with some changes. The anthocyanin content was measured by (Mita et al., 1997) method. Results: The Methanolic extract of aerial parts exhibited slight antibacterial effects against B.cereus at the concentration of 3 mg/disc. The IC50 values for Methanolic, Dichloromethan and Hexane extracts in DPPH assay were 10.071 ± 1.529, 8.756 ± 0.206 and 29.551 ± 6.457 mg/ml, respectively. The phenolic content of the Methanolic, Dichloromethan and Hexane extracts were obtained 56.66, 14 and 11 mg/ml, respectively. The total flavonoid content of the Methanolic, Dichloromethan and Hexane extracts were obtained 22, 84.15 and 51.9, respectively. Anthocyanin amount, contained in one gram sample of dried aerial Cyperus longus was 11 mg. Conclusion: Antibacterial test results were showed that it has no effect on Gram-negative bacteria. But it is effective on Gram-positive bacteria. All extracts showed antioxidant activity in DPPH method and the Dichloromethan and Hexane extracts were found to have maximum and minimum activity in DPPH assay, respectively.