Journal of North Khorasan

Background & objectives: Diazinon (DZN) is an OP insecticide that one of its main mechanisms of toxicity is the inhibition of cholinesterase. The aim of the present study was to investigate the effects of diazinon on cholinesterase activity in adult female rat and protective role of vitamin E that performed following other study on diazinon-induced ovary oxidative stress.

Material & Methods:  In total 30 adult female wistar rats were divided into 5 groups: control (without any intervention), placebo (received only pure olive oil), experimental 1 (DZN+olive oil, 60 mg/kg), experimental 2 (DZN, 60 mg/kg +Vit E, 200 mg/kg) and experimental 3 (Vit E, 200 mg/kg). After 2 weeks, 3ml blood from the heart was collected with a heparinated syringe. The Serum and erythrocytes cholinesterases inhibition activity was determined.

Results: The results revealed that DZN reduces AchE level in erythrocyte significantly in comparison with those of control group (p< 0.001). Also DZN reduced PchE level in serum significantly (p< 0.05). In addition, Vit E plus DZN increased AchE level in erythrocyte significantly in comparison with those of DZN group (p< 0.001) . A significant increase in PchE level was observed   (p< 0.05).

Conclusion: The findings of this study indicate DZN decreases AchE and PchE levels in blood while in contrast  use of vit E, increase their  levels. Therefore, it can be concluded that vit E as antioxidants has a protective effect on AchE activity and reduce the toxicity of DZN in adult female rats.

Keyword: Diazinon, erythrocyte cholinesterase , Rat,  Serum cholinesterase, Vitamin E