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JNKUMS 2018, 9(4): 34-41 Back to browse issues page
Comparison of Precipitation Reference Method with New Direct Assay of Total Iron Binding Capacity (TIBC)
Shadi Sharifi , Mohammad Javadzadeh , Ali Sherafatnia , Ghorbani , Elham Sharifian , Amirhosein Khoshi
Abstract:   (776 Views)
Introduction: Total iron binding capacity (TIBC) assay is important investigation of
body iron state. The most common method is chemical-precipitation reference method
with pre-analysis step. New diagnostic approaches for direct measurement of TIBC have
been proposed to streamline and reducing the time of analysis. The aim of study was to
compare of accuracy and precision of TIBC direct assay with precipitation method.
Methods: Under standard laboratory condition, twenty-five individuals' serum samples
for each of the low, normal and high TIBC levels were analyzed by both precipitation
and direct methods. To investigate of analytical accuracy and precision, the assayedcommercial
serum-control was used.
Results: There were not significant difference between different serum-TIBC levels
which analyzed by precipitation and direct methods (P=0.25, P=0.17 and P=0.34 for low,
normal and high levels, respectively); however, there were more differences between
serum-TIBC that analyzed by precipitation method. To investigate of within-run
9%) for
precipitation and direct methods, respectively. However, there were not significant
differences in accuracy and precision between two methods (P=0.53).
Conclusions: The results showed that despite of allowable accuracy and no significant
differences between precipitation and direct methods of TIBC measurement, because of
lower standard deviation and CV%, and also simplicity of direct assay, it is suggested
that the direct assay can be replace instead of precipitation method.
Keywords: Total Iron Binding Capacity (TIBC) Chemical precipitation Direct assay Precision Accuracy
Full-Text [PDF 1138 kb]   (908 Downloads)    
Type of Study: Orginal Research | Subject: Basic Sciences
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
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Sharifi S, Javadzadeh M, Sherafatnia A, Ghorbani, Sharifian E, Khoshi A. Comparison of Precipitation Reference Method with New Direct Assay of Total Iron Binding Capacity (TIBC). JNKUMS. 2018; 9 (4) :34-41
URL: http://journal.nkums.ac.ir/article-1-1311-en.html

Volume 9, Issue 4 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی خراسان شمالی Journal of North Khorasan University of Medical Sciences
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