Volume 9, Issue 4 (3-2018)
2018, 9(4): 34-41 |
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Sharifi S, Javadzadeh M, Sherafatnia A, Ghorbani, Sharifian E, Khoshi A. Comparison of Precipitation Reference Method with New Direct Assay of Total Iron Binding Capacity (TIBC). North Khorasan University of Medical Sciences 2018; 9 (4) :34-41
URL: http://journal.nkums.ac.ir/article-1-1311-en.html
URL: http://journal.nkums.ac.ir/article-1-1311-en.html
Shadi Sharifi1 
, Mohammad Javadzadeh2 , Ali Sherafatnia3 , Ghorbani , Elham Sharifian4 , Amirhosein Khoshi * 5
, Mohammad Javadzadeh2 , Ali Sherafatnia3 , Ghorbani , Elham Sharifian4 , Amirhosein Khoshi * 5
1- Clinical Laboratory B.Sc., Student Research Committee, North Khorasan University of Medical Sciences, Bojnurd, Iran
2- Clinical Laboratory of Imam Reza Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Epidemiology M.Sc., Department of Social Medicine, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Assistant professor of Clinical Biochemistry, Ph.D., Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant professor of Clinical Biochemistry, Ph.D., Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , ah.khoshi@nkums.ac.ir
2- Clinical Laboratory of Imam Reza Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Epidemiology M.Sc., Department of Social Medicine, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Assistant professor of Clinical Biochemistry, Ph.D., Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant professor of Clinical Biochemistry, Ph.D., Department of Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran , ah.khoshi@nkums.ac.ir
Abstract: (3998 Views)
Introduction: Total iron binding capacity (TIBC) assay is important investigation of body iron state. The most common method is chemical-precipitation reference method with pre-analysis step. New diagnostic approaches for direct measurement of TIBC have been proposed to streamline and reducing the time of analysis. The aim of study was to compare of accuracy and precision of TIBC direct assay with precipitation method.
Methods: Under standard laboratory condition, twenty-five individuals' serum samples for each of the low, normal and high TIBC levels were analyzed by both precipitation and direct methods. To investigate of analytical accuracy and precision, the assayedcommercial serum-control was used.
Results: There were not significant difference between different serum-TIBC levels which analyzed by precipitation and direct methods (P=0.25, P=0.17 and P=0.34 for low, normal and high levels, respectively); however, there were more differences between serum-TIBC that analyzed by precipitation method. To investigate of within-run -run9%) for precipitation and direct methods, respectively. However, there were not significant differences in accuracy and precision between two methods (P=0.53).
Conclusions: The results showed that despite of allowable accuracy and no significant differences between precipitation and direct methods of TIBC measurement, because of lower standard deviation and CV%, and also simplicity of direct assay, it is suggested that the direct assay can be replace instead of precipitation method.
Methods: Under standard laboratory condition, twenty-five individuals' serum samples for each of the low, normal and high TIBC levels were analyzed by both precipitation and direct methods. To investigate of analytical accuracy and precision, the assayedcommercial serum-control was used.
Results: There were not significant difference between different serum-TIBC levels which analyzed by precipitation and direct methods (P=0.25, P=0.17 and P=0.34 for low, normal and high levels, respectively); however, there were more differences between serum-TIBC that analyzed by precipitation method. To investigate of within-run -run9%) for precipitation and direct methods, respectively. However, there were not significant differences in accuracy and precision between two methods (P=0.53).
Conclusions: The results showed that despite of allowable accuracy and no significant differences between precipitation and direct methods of TIBC measurement, because of lower standard deviation and CV%, and also simplicity of direct assay, it is suggested that the direct assay can be replace instead of precipitation method.
Keywords: Total Iron Binding Capacity(TIBC), Chemical precipitation, Direct assay, Precision, Accuracy
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
Received: 2018/03/18 | Accepted: 2018/03/18 | Published: 2018/03/18
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