Volume 16, Issue 1 (Spring 2024)
2024, 16(1): 1-11 |
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Ethics code: IR.MUMS.REC.1395.272
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Mohammadi M, Babaei Mahani M, Ramezani M, Alibolandi M. Collagen-Hydroxyapatite Scaffold Platform Incorporating Dimethyloxalylglycine Loaded Polymersomes Decorated with BMP-2 Peptide for Bone Tissue Engineering. North Khorasan University of Medical Sciences 2024; 16 (1) :1-11
URL: http://journal.nkums.ac.ir/article-1-2919-en.html
URL: http://journal.nkums.ac.ir/article-1-2919-en.html
1- Assistant Professor, Department of Pharmaceutics, School of pharmacy and Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2- Post Doctorate, Central Research Laboratory, Mashhad University of Medical Sciences, Mashhad, Iran
3- Distinguished Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4- Associate Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran ,alibolandim@mums.ac.ir
2- Post Doctorate, Central Research Laboratory, Mashhad University of Medical Sciences, Mashhad, Iran
3- Distinguished Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4- Associate Professor, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran ,
Abstract: (932 Views)
Introduction: Recent advances in the field of nanotechnology have led to the development of platforms for healing or repairing damaged tissues. The present study aimed to provide a bone extracellular matrix (ECM) mimetic platform made of hydroxyapatite and collagen loaded with nanopolymersomes encapsulating dimethyloxalylglycine (DMOG) and decorated with BMP-2 peptide in order to accelerate and increase the differentiation of mesenchymal stem cells to bone tissue.
Method: In this study, polymersome nanoparticles carrying DMOG and targeted with BMP-2 peptide were prepared. The physicochemical properties of nanoparticles were evaluated. Thereafter, it was loaded into collagen-nanohydroxyapatite scaffolds and the expression levels of osteogenic genes OCN and OPN and angiogenic genes vcam, vwf, and kdr were checked in mesenchymal stem cells.
Results: The surface charge and particle size of the synthesized nanoparticles were reported as -6 mv and 141 nm, respectively. The nanoparticles were able to slowly release 35% of the loaded DMOG within 30 days. The results demonstrated that the expression levels of OCN and OPN genes in cells exposed to polymersomes targeted with BMP-2 peptide were 27 and 2 times more than those of non-targeted polymersomes, respectively. In addition, the expression of angiogenic genes, vwf, kdr, and vcam, displayed a significant increase in targeted polymersomes compared to the non-targeted types (11.7-, 12.8-, and 2.17-fold, respectively).
Conclusion: It seems that providing an environment similar to the bone ECM by combining an osteomimetic scaffold made of collagen-nanohydroxyapatite, DMOG as an angiogenic factor, and BMP-2 as the osteogenic factor can be effective in accelerating and increasing the differentiation of mesenchymal stem cells into osteoblasts.
Method: In this study, polymersome nanoparticles carrying DMOG and targeted with BMP-2 peptide were prepared. The physicochemical properties of nanoparticles were evaluated. Thereafter, it was loaded into collagen-nanohydroxyapatite scaffolds and the expression levels of osteogenic genes OCN and OPN and angiogenic genes vcam, vwf, and kdr were checked in mesenchymal stem cells.
Results: The surface charge and particle size of the synthesized nanoparticles were reported as -6 mv and 141 nm, respectively. The nanoparticles were able to slowly release 35% of the loaded DMOG within 30 days. The results demonstrated that the expression levels of OCN and OPN genes in cells exposed to polymersomes targeted with BMP-2 peptide were 27 and 2 times more than those of non-targeted polymersomes, respectively. In addition, the expression of angiogenic genes, vwf, kdr, and vcam, displayed a significant increase in targeted polymersomes compared to the non-targeted types (11.7-, 12.8-, and 2.17-fold, respectively).
Conclusion: It seems that providing an environment similar to the bone ECM by combining an osteomimetic scaffold made of collagen-nanohydroxyapatite, DMOG as an angiogenic factor, and BMP-2 as the osteogenic factor can be effective in accelerating and increasing the differentiation of mesenchymal stem cells into osteoblasts.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2023/11/4 | Accepted: 2023/11/15 | Published: 2024/03/26
Received: 2023/11/4 | Accepted: 2023/11/15 | Published: 2024/03/26
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