Volume 18, Issue 1 (Spring 2026)
2026, 18(1): 7-15 |
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Miri M A, Daneshmand S, Shahraki J, Shahraki O. Evaluation of the Wound Healing Effect of Nanofibers Enriched with Eucalyptus Extract and Flaxseed Oil in Mice. North Khorasan University of Medical Sciences 2026; 18 (1) :7-15
URL: http://journal.nkums.ac.ir/article-1-3345-en.html
URL: http://journal.nkums.ac.ir/article-1-3345-en.html
1- Department of Food Sciences and Technology, University of Zabol, Zabol, Iran
2- Department of Pharmaceutics, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran ,sdmehrpooya@gmail.com
3- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
4- Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran
2- Department of Pharmaceutics, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran ,
3- Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran
4- Infectious Diseases and Tropical Medicine Research Center, Research Institute of Cellular and Molecular Sciences in Infectious Diseases, Zahedan University of Medical Sciences, Zahedan, Iran
Abstract: (176 Views)
Introduction: The treatment of wounds has long been one of the challenges faced by humanity. Therefore, efforts to discover compounds that promote wound healing with fewer side effects are essential. Eucalyptus and flaxseed are plant-derived products, the antimicrobial and wound healing effects of which have been reported in many studies. Recently, the electrospinning process has been widely used as a technology for the production of nanofibers that can provide conditions similar to the extracellular matrix. The present study aimed to evaluate the effect of nanofibers containing eucalyptus and flaxseed oil on skin wound healing in mice.
Methods: During the process of preparing nanofibers, eucalyptus essential oil and flaxseed oil were loaded into the nanofibers prepared from Zein. Afterwards, to investigate the effect on wound healing, 36 mice were divided into 6 groups: negative control (distilled water), positive control (alpha ointment), blank (nanofibers without eucalyptus essential oil and flaxseed oil), nanofibers containing flaxseed oil (Fiber-Flex), nanofibers containing eucalyptus essential oil (Fiber-Euc), and nanofibers containing eucalyptus essential oil and flaxseed oil (Fiber-Euc-Flex). After creating a wound in all groups, the products were used on the wound site for 14 days. For the macroscopic study, wound size was measured on days 0, 1, 2, 3, 5, 7, 9, 11, 13, and 14. Moreover, on the 14th day after wounding, the mice were euthanized, and the repaired tissues were examined for histopathological studies. Hematoxylin-eosin staining was performed on the samples and examined by a pathologist. Data analysis was performed using SPSS software (version 25.0)
Results: On the second day, the mean wound diameter in the Fiber-Euc-Flex group was lower than that of the negative control group. The mean diameter of the wound on the 7th, 9th, 11th, and 14th day in the Fiber-Euc-Flex group was significantly lower than that of the negative control group. On the 13th day of the study, the mean diameter of the wound in all the investigated groups was lower than that of the negative control group. On the 14th, the mean diameter of the wound in the Fiber-Euc-Flex group was significantly lower than that in all other groups.
Conclusions: The results of the current study showed that nanofibers containing eucalyptus and flaxseed oil accelerated the wound healing process and reduced the time required for complete wound healing.
Methods: During the process of preparing nanofibers, eucalyptus essential oil and flaxseed oil were loaded into the nanofibers prepared from Zein. Afterwards, to investigate the effect on wound healing, 36 mice were divided into 6 groups: negative control (distilled water), positive control (alpha ointment), blank (nanofibers without eucalyptus essential oil and flaxseed oil), nanofibers containing flaxseed oil (Fiber-Flex), nanofibers containing eucalyptus essential oil (Fiber-Euc), and nanofibers containing eucalyptus essential oil and flaxseed oil (Fiber-Euc-Flex). After creating a wound in all groups, the products were used on the wound site for 14 days. For the macroscopic study, wound size was measured on days 0, 1, 2, 3, 5, 7, 9, 11, 13, and 14. Moreover, on the 14th day after wounding, the mice were euthanized, and the repaired tissues were examined for histopathological studies. Hematoxylin-eosin staining was performed on the samples and examined by a pathologist. Data analysis was performed using SPSS software (version 25.0)
Results: On the second day, the mean wound diameter in the Fiber-Euc-Flex group was lower than that of the negative control group. The mean diameter of the wound on the 7th, 9th, 11th, and 14th day in the Fiber-Euc-Flex group was significantly lower than that of the negative control group. On the 13th day of the study, the mean diameter of the wound in all the investigated groups was lower than that of the negative control group. On the 14th, the mean diameter of the wound in the Fiber-Euc-Flex group was significantly lower than that in all other groups.
Conclusions: The results of the current study showed that nanofibers containing eucalyptus and flaxseed oil accelerated the wound healing process and reduced the time required for complete wound healing.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2023/11/11 | Accepted: 2025/12/23 | Published: 2026/03/29
Received: 2023/11/11 | Accepted: 2025/12/23 | Published: 2026/03/29
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