Volume 18, Issue 1 (Spring 2026)
2026, 18(1): 16-24 |
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Ethics code: IR.IAU.DAMGHAN.REC.1403.025
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Eyvazloo H, Kahrizi F, Tabeshpour J. Investigation of the Protective Effect of Garlic on Acrylamide-Induced Neurotoxicity via the Apoptotic Pathway in Male Wistar Rats. North Khorasan University of Medical Sciences 2026; 18 (1) :16-24
URL: http://journal.nkums.ac.ir/article-1-3346-en.html
URL: http://journal.nkums.ac.ir/article-1-3346-en.html
1- Department of Pharmacology, Da.C., Islamic Azad University, Damghan, Iran
2- Department of Pharmacology, Da.C., Islamic Azad University, Damghan, Iran ,jamshid.tabeshpour@iau.ac.ir
2- Department of Pharmacology, Da.C., Islamic Azad University, Damghan, Iran ,
Abstract: (170 Views)
Introduction: Acrylamide, a neurotoxic compound, enters human life through various sources. Garlic, with its antioxidant properties, protects the nervous system. This study aimed to investigate the effect of garlic on reducing acrylamide-induced neurotoxicity in male Wistar rats by evaluating the apoptosis pathway and measuring the levels of BAX, Bcl-2, and caspase-3 proteins.
Methods: Male Wistar rats were divided into seven groups (n=6 per group): Group 1 received normal saline, Group 2 received acrylamide (50 mg/kg), Groups 3–5 received acrylamide + garlic (100, 200, and 400 mg/kg, respectively), Group 6 received only garlic (200 mg/kg), and Group 7 received acrylamide + vitamin E (200 mg/kg). Intraperitoneal injections were administered for 11 days. Subsequently, the rats' cerebral cortex was extracted and analyzed via Western blot. The densitometric data for protein bands are expressed as mean ± standard deviation (Mean ± SD). Intergroup comparisons were performed using one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. All statistical analyses were conducted using GraphPad Prism software (version 9.4.1). A P-value of less than 0.05 was considered statistically significant.
Results: The findings revealed that acrylamide alone increased the levels of caspase-3 and Bcl-2. Garlic at 100 mg/kg significantly elevated Bcl-2, compared to the normal and acrylamide-only groups; however, it did not significantly affect caspase-3 levels. The 400 mg/kg garlic dose showed the highest increase in Bcl-2 levels. Vitamin E also reduced caspase-3 and increased Bcl-2, though no significant difference was observed, compared to the 100 mg/kg garlic group.
Conclusions: Intraperitoneal injection of acrylamide in male Wistar rats induced apoptosis. However, Western blot results demonstrated that treatment with different doses of garlic prevented apoptosis and reduced neural damage.
Methods: Male Wistar rats were divided into seven groups (n=6 per group): Group 1 received normal saline, Group 2 received acrylamide (50 mg/kg), Groups 3–5 received acrylamide + garlic (100, 200, and 400 mg/kg, respectively), Group 6 received only garlic (200 mg/kg), and Group 7 received acrylamide + vitamin E (200 mg/kg). Intraperitoneal injections were administered for 11 days. Subsequently, the rats' cerebral cortex was extracted and analyzed via Western blot. The densitometric data for protein bands are expressed as mean ± standard deviation (Mean ± SD). Intergroup comparisons were performed using one-way analysis of variance (ANOVA), followed by Tukey's post hoc test. All statistical analyses were conducted using GraphPad Prism software (version 9.4.1). A P-value of less than 0.05 was considered statistically significant.
Results: The findings revealed that acrylamide alone increased the levels of caspase-3 and Bcl-2. Garlic at 100 mg/kg significantly elevated Bcl-2, compared to the normal and acrylamide-only groups; however, it did not significantly affect caspase-3 levels. The 400 mg/kg garlic dose showed the highest increase in Bcl-2 levels. Vitamin E also reduced caspase-3 and increased Bcl-2, though no significant difference was observed, compared to the 100 mg/kg garlic group.
Conclusions: Intraperitoneal injection of acrylamide in male Wistar rats induced apoptosis. However, Western blot results demonstrated that treatment with different doses of garlic prevented apoptosis and reduced neural damage.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2025/06/11 | Accepted: 2025/11/4 | Published: 2026/03/29
Received: 2025/06/11 | Accepted: 2025/11/4 | Published: 2026/03/29
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