Volume 18, Issue 2 (Summer 2026)
2026, 18(2): 102-110 |
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Ethics code: IR.IAU.ARAK.REC.1403.259
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Nazariyeh E, Abedi B, Moradi M, Maleki Pouya M. The Effect of an Endurance Training Combined with Electrical Stimulation on the Expression of TNF-α and IL-6 Genes in the Adipose Tissue of Obese Fasting Rats. North Khorasan University of Medical Sciences 2026; 18 (2) :102-110
URL: http://journal.nkums.ac.ir/article-1-3389-en.html
URL: http://journal.nkums.ac.ir/article-1-3389-en.html
1- PhD Candidate, Department of Exercise Physiology, Mahalat Branch, Islamic Azad University, Mahallat, Iran
2- Professor, Department of Exercise Physiology, North Tehran Branch, Islamic Azad University, Tehran, Iran ,bahram.Abedi@iau.ac.ir
3- Assistant Professor, Department of Exercise Physiology, Arak Branch, Islamic Azad University, Arak, Iran
2- Professor, Department of Exercise Physiology, North Tehran Branch, Islamic Azad University, Tehran, Iran ,
3- Assistant Professor, Department of Exercise Physiology, Arak Branch, Islamic Azad University, Arak, Iran
Abstract: (41 Views)
Introduction: The present study aimed to investigate the effects of endurance training combined with electrical stimulation on the gene expression of IL-6 and TNF-α in the adipose tissue of fasted obese mice.
Methods: In this experimental study, 35 rats were randomly divided into five groups of seven after obesity induction. Fasting was performed as a 16/8 protocol for all groups except the obese control. The endurance training protocol consisted of four weeks and five sessions per week, with an intensity of 50% of maximum oxygen consumption in the first week, increasing to 65% in the fourth week. Electrical stimulation was also performed using a foot-shock device. The expression levels of IL-6 and TNF-α in adipose tissue were measured 24 hours after the last training session using real-time PCR. One-way analysis of variance with Tukey's post hoc test in SPSS (version 23) software was used to analyze the data.
Results: IL-6 gene expression levels in the F.EX.ES group were significantly increased compared to the (CO) (P=0.001) and (F.ES) groups (P=0.001). Also, a significant increase was observed in the F.EX.ES group compared with the F (P=0.047) and F.EX (P=0.049) groups. TNF-α gene expression was also significantly higher in the F.EX, F.ES, and F.EX.ES groups than in the CO group (P=0.001). Also, TNF-α gene expression in the F.EX group was significantly higher than in the F group (P=0.001). TNF-α levels in the F.ES (P=0.032) and F.EX.ES (P=0.015) groups significantly increased compared with the F group.
Conclusions: It appears that obesity increases proinflammatory cytokine levels, and performing endurance training and fasting according to the protocol of the present study can positively affect cytokine levels.
Methods: In this experimental study, 35 rats were randomly divided into five groups of seven after obesity induction. Fasting was performed as a 16/8 protocol for all groups except the obese control. The endurance training protocol consisted of four weeks and five sessions per week, with an intensity of 50% of maximum oxygen consumption in the first week, increasing to 65% in the fourth week. Electrical stimulation was also performed using a foot-shock device. The expression levels of IL-6 and TNF-α in adipose tissue were measured 24 hours after the last training session using real-time PCR. One-way analysis of variance with Tukey's post hoc test in SPSS (version 23) software was used to analyze the data.
Results: IL-6 gene expression levels in the F.EX.ES group were significantly increased compared to the (CO) (P=0.001) and (F.ES) groups (P=0.001). Also, a significant increase was observed in the F.EX.ES group compared with the F (P=0.047) and F.EX (P=0.049) groups. TNF-α gene expression was also significantly higher in the F.EX, F.ES, and F.EX.ES groups than in the CO group (P=0.001). Also, TNF-α gene expression in the F.EX group was significantly higher than in the F group (P=0.001). TNF-α levels in the F.ES (P=0.032) and F.EX.ES (P=0.015) groups significantly increased compared with the F group.
Conclusions: It appears that obesity increases proinflammatory cytokine levels, and performing endurance training and fasting according to the protocol of the present study can positively affect cytokine levels.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2025/07/1 | Accepted: 2026/01/5 | Published: 2026/07/1
Received: 2025/07/1 | Accepted: 2026/01/5 | Published: 2026/07/1
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