Journal of North Khorasan

Abstract Background & Objectives:The modern essence extraction methods are following special patterns in order to increase the quality, prevent the contamination, and decrease the sample preparation costs. In order to get the best essence possible, subjects like the herbal components properties, suitable solvent and precision should be put into serious consideration. In this current study we have compared the Biebersteinia multifida DC essential oils obtained with two different solvents and methods, hydro distillation and normal hexane extraction. Material and Methods:After collecting the plant sample, and conducting the drying procedure, the essential oils were obtained using two various methods, solvent extraction and the hydro distillation (Clevenger). Later the essential oil components were analyzed using GC, MASS SPECTROSCOPY devices. Furthermore the yield of extraction was also studied for both of the mentioned methods. Results:In hydro distillation protocol and solvent extraction method 30 and 13 compounds were recognized respectively. On the other hand the optimum time for the Clevenger method was calculated to be 360 minutes however it was 3 days for solvent extraction method. The most profound advantages about the hydro distillation method can be its short duration, high extraction yield and using the non toxic-environmental friendly solvent. Conclusion: Selecting the most proper method should be based on the desired compound. The extraction yield for the Clevenger method was 1.33 times more than the solvent extraction method.