Journal of North Khorasan

Background & Objectives: Madder perennial herb or Rubia florida is an industrial plant which is mainly used as a dye in the past time. The purpose of this study was to investigate  the essential active compounds in Rubia florida oil. That is native in North Khorasan province and this project paves the way for future pharmaceutical researches and applications.                                                                                 

Materials and Methods: Samples were collected and extracted by water distillation method after drying at room temperature. The essential oils yield was %0.031. Components of essential oil were studied with gas chromatography mass spectrometer (GC/MS). Identification of essential oil components was done using a gas chromatography which was connected to a mass spectrometer GC- MASS. Identification of essential constituent compounds was done using the retention indices and mass spectra of compounds and comparing them with standard mass spectra in the computer libraries.

Results: The yields of aerial parts of Rubia florida was 031.0% and 32 compound were identified in essential oil. The major compounds such as Decane (8.38%), Palmitic acid (8.07%), octane (% 6.55), Palmitic acid methyl ester (% 5.11), linoleic acid, ethyl esther (% 5.53) were identified in the essential oil.

Conclusion: Researchers demonstrated the antioxidant and anti-cancer activity and prevention of food allergy in the cis and trans isomers of linoleic acid. Due to the composition it was concluded that the 52.12% of extracts of Rubia florida had antioxidant compounds.