Journal of North Khorasan

Abstract Background and Objectives: Most housekeeping genes, tumor-suppressor genes, and approx 40% of tissue specific genes contain G+C sequences in their promoter region that are very difficult to amplify. AEG-1 plays a significant role in invasion, metastasis, angiogenesis and chemoresistance, apoptosis, angiogenesis and aging Material and Methods:In this study, we proposed an improved polymerase chain reaction (PCR) method to be used for successful amplification of the Astrocyte elevated gene -1gene promoter region that contains >70% G+C content in a sequence of 495 bp and some transcription initiation sites in hepatocellularcarcinoma patient. Results: The results showed that using of DMSO co-amplification touchdown PCR and pfu enzyme can inhibit the formation of secondary structure in DNA Sequences and increase the PCR product. Conclusion: Therefore, this method can be recommended to amplify other GC-rich genomic templates.