Volume 11, Issue 1 (5-2019)
2019, 11(1): 51-57 |
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Shafie B, Pourahmad J, Rezaei M. The Effect of Resveratrol against Acrolein Toxicity in Mitochondria isolated from Rat Liver. North Khorasan University of Medical Sciences 2019; 11 (1) :51-57
URL: http://journal.nkums.ac.ir/article-1-1729-en.html
URL: http://journal.nkums.ac.ir/article-1-1729-en.html
1- MSc Student of Toxicology, Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Professor of Toxicology and Pharmacology, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Assistant Professor of Toxicology, Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Professor of Toxicology and Pharmacology, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Assistant Professor of Toxicology, Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract: (3668 Views)
Introduction: Acrolein is an important environmental, food and water pollutant that plays an important role in the development of several diseases. Resveratrol is a phenolic compound with antioxidant properties found in fruits such as grapes, berries and peanuts. The purpose of this study was to evaluate the protective effect of resveratrol in preventing Acrolein-induced toxicity in isolated mitochondria from the rat liver. Methods: Rat liver mitochondria were isolated by differential centrifugation. Different concentrations of Acrolein (100 μM to 2000 μM) were used to obtain an IC50. Different concentrations of resveratrol (2.5 μM to 40 μM) were then applied on mitochondria in the presence of Acrolein toxicity.
Results: Acrolein IC50 was obtained via MTT assay as 400 μM. No toxic effect was observed for resveratrol and in the presence of Acrolein, there was a significant reduction in mitochondrial survival even at high concentrations of resveratrol compared to the control group (P < 0.001).
Conclusions: The results of these study showed that resveratrol failed to exhibit a protective effect against acute Acrolein toxicity and just because a protective substance could be a marvelous antioxidant does not mean that it can prevent the mitochondrial damage resulted from any toxicant.
Results: Acrolein IC50 was obtained via MTT assay as 400 μM. No toxic effect was observed for resveratrol and in the presence of Acrolein, there was a significant reduction in mitochondrial survival even at high concentrations of resveratrol compared to the control group (P < 0.001).
Conclusions: The results of these study showed that resveratrol failed to exhibit a protective effect against acute Acrolein toxicity and just because a protective substance could be a marvelous antioxidant does not mean that it can prevent the mitochondrial damage resulted from any toxicant.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2018/04/24 | Accepted: 2018/10/29 | Published: 2019/06/18
Received: 2018/04/24 | Accepted: 2018/10/29 | Published: 2019/06/18
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