Volume 17, Issue 3 (Autumn 2025)
2025, 17(3): 45-53 |
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Vahidi N, Mohammadi A, Mollazadeh H, Teymouri M, Alesheikh P, rameshrad M et al . Effect of Ethanolic Extract of Dracocephalum Kotschyi on Inflammatory and Oxidative Stress Biomarkers in Male Wistar Rats. North Khorasan University of Medical Sciences 2025; 17 (3) :45-53
URL: http://journal.nkums.ac.ir/article-1-3264-en.html
URL: http://journal.nkums.ac.ir/article-1-3264-en.html
Nafiseh Vahidi1 
, Ameneh Mohammadi2 , Hamid Mollazadeh3 , Manouchehr Teymouri4 , Peiman Alesheikh4 , Maryam Rameshrad *5 , Jamal Kasaian4
, Ameneh Mohammadi2 , Hamid Mollazadeh3 , Manouchehr Teymouri4 , Peiman Alesheikh4 , Maryam Rameshrad *5 , Jamal Kasaian4
1- Student Research Committee, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
2- MSc in Phytochemistry, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Assistant Professor, Department of Physiology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Assistant Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran. Assistant Professor, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ,mrameshrad@gmail.com
2- MSc in Phytochemistry, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
3- Assistant Professor, Department of Physiology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4- Assistant Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
5- Assistant Professor, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran. Assistant Professor, Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran ,
Abstract: (264 Views)
Introduction: Oxidative stress is a phenomenon caused by an imbalance between the production and accumulation of reactive oxygen species (ROS) in cells and tissues, and the inability of a biological system to detoxify these reactive products. Dracocephalum kotschyi is traditionally used in local treatment for inflammatory complications. This study investigated the effect of D. kotschyi extract on oxidative stress biomarkers in male Wistar rats.
Method: Male Wistar rats were randomly divided into five groups, each with ten rats, and received ethanol extract (12.5, 25, and 50 mg/kg, i.p.) and indomethacin (2.5 mg/kg) as the reference drug. Rat hind paw edema caused by carrageenan injection was used for acute inflammation. The animals were inoculated with carrageenan (1% w/v, 0.1 ml) before measuring the thickness of their left hind paw at hourly intervals. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined using standard methods. Moreover, the major bioactive compound in the extract was identified using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy. In addition, comparisons were made using one-way analysis of variance (ANOVA).
Results: The edema reduction with the extract was comparable to that of indomethacin, especially at 12.5 and 25 mg/kg doses, from the very beginning of carrageenan inoculation (p<0.01). The extract also remarkably reduced the level of MDA (p<0.001), enhanced the activity of ROS-scavenging enzymes (p<0.001), and exhibited a significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. Moreover, 1 g of the extract yielded 23.8 mg of luteolin-7-O-glucoside following purification. In conclusion, D. kotschyi ethanol extract is a potential herbal medicine for treating edema and ROS reactions associated with it, likely due to the presence of effective phenolic compounds, such as luteolin-7-O-glucoside.
Conclusion: The ethanol extract of D. kotschyi could be a potential herbal medicine for the treatment of edema and its resulting ROS reactions, likely due to the presence of compounds such as luteolin-7-o-glucoside.
Method: Male Wistar rats were randomly divided into five groups, each with ten rats, and received ethanol extract (12.5, 25, and 50 mg/kg, i.p.) and indomethacin (2.5 mg/kg) as the reference drug. Rat hind paw edema caused by carrageenan injection was used for acute inflammation. The animals were inoculated with carrageenan (1% w/v, 0.1 ml) before measuring the thickness of their left hind paw at hourly intervals. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined using standard methods. Moreover, the major bioactive compound in the extract was identified using high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) spectroscopy. In addition, comparisons were made using one-way analysis of variance (ANOVA).
Results: The edema reduction with the extract was comparable to that of indomethacin, especially at 12.5 and 25 mg/kg doses, from the very beginning of carrageenan inoculation (p<0.01). The extract also remarkably reduced the level of MDA (p<0.001), enhanced the activity of ROS-scavenging enzymes (p<0.001), and exhibited a significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity. Moreover, 1 g of the extract yielded 23.8 mg of luteolin-7-O-glucoside following purification. In conclusion, D. kotschyi ethanol extract is a potential herbal medicine for treating edema and ROS reactions associated with it, likely due to the presence of effective phenolic compounds, such as luteolin-7-O-glucoside.
Conclusion: The ethanol extract of D. kotschyi could be a potential herbal medicine for the treatment of edema and its resulting ROS reactions, likely due to the presence of compounds such as luteolin-7-o-glucoside.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2025/04/13 | Accepted: 2025/06/21 | Published: 2025/09/29
Received: 2025/04/13 | Accepted: 2025/06/21 | Published: 2025/09/29
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