Volume 18, Issue 1 (Spring 2026)
2026, 18(1): 69-77 |
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Ethics code: IR.IAU.BOJNOURD.REC.1403.008
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Rahmani Z, Rezaeian N, Cheragh Birjandi S, Ya’ghoubi A, Rezaei V. Effect of High Intensity Interval Training on Visceral Adipose Tissue Levels of Isthmin-1 and Insulin Resistance in Obese Diabetic Rat. North Khorasan University of Medical Sciences 2026; 18 (1) :69-77
URL: http://journal.nkums.ac.ir/article-1-3352-en.html
URL: http://journal.nkums.ac.ir/article-1-3352-en.html
1- PhD Candidate in Exercise Physiology, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran
2- PhD in Exercise Physiology, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran ,rezaeian@bojnourdiau.ac.ir
3- PhD in Exercise Physiology, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran
4- PhD in Sport Injuries and Corrective Exercises, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran
2- PhD in Exercise Physiology, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran ,
3- PhD in Exercise Physiology, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran
4- PhD in Sport Injuries and Corrective Exercises, Assistant Professor, Department of Physical Education, Boj.C., Islamic Azad University, Bojnord, Iran
Abstract: (127 Views)
Introduction: Isthmin-1 is a novel adipokine with anti-inflammatory functions that plays a role in regulating glucose metabolism and the incidence of type 2 diabetes. This study aimed to investigate the effect of eight weeks of high-intensity interval training (HIIT) on adipose tissue levels of Isthmin-1 and the insulin resistance index (HOMA-IR) in obese diabetic rats.
Methods: 20 male Wistar rats (10-12 weeks old, 276.4±11.20 g) were randomly divided into two groups of experimental and control (n=10 per group), following the induction of obesity and diabetes via a high-fat diet and injection of Streptozotocin. Rats in the experimental group participated in eight weeks of HIIT, running on a treadmill at intensity of 85%-90% of maximum velocity, 6-12 sets with 1-minute rest intervals, five days per week. All rats were dissected after the last training session, and the relevant indicators were evaluated. Data analysis was performed using independent and paired t-tests, as well as Pearson's correlation test, with the significance level set at P < 0.05.
Results: Eight weeks of HIIT resulted in significant decreases in insulin levels (P=0.007), HOMA-IR (P=0.032), and body weight (P=0.029), alongside a significant increase in adipose tissue levels of Isthmin-1 (P=0.006) in the experimental group, compared to the control group. Moreover, fasting blood sugar (P=0.024) and body weight (P=0.005) showed significant decreases in the experimental group in the post-test, compared to the pre-test. Furthermore, a significant correlation was observed between the changes in Isthmin-1 levels after eight weeks of HIIT and the changes in insulin levels (P=0.014).
Conclusions: It appears that eight weeks of HIIT modulates insulin resistance by increasing Isthmin-1 levels in obese diabetic rats, in addition to improving body composition.
Methods: 20 male Wistar rats (10-12 weeks old, 276.4±11.20 g) were randomly divided into two groups of experimental and control (n=10 per group), following the induction of obesity and diabetes via a high-fat diet and injection of Streptozotocin. Rats in the experimental group participated in eight weeks of HIIT, running on a treadmill at intensity of 85%-90% of maximum velocity, 6-12 sets with 1-minute rest intervals, five days per week. All rats were dissected after the last training session, and the relevant indicators were evaluated. Data analysis was performed using independent and paired t-tests, as well as Pearson's correlation test, with the significance level set at P < 0.05.
Results: Eight weeks of HIIT resulted in significant decreases in insulin levels (P=0.007), HOMA-IR (P=0.032), and body weight (P=0.029), alongside a significant increase in adipose tissue levels of Isthmin-1 (P=0.006) in the experimental group, compared to the control group. Moreover, fasting blood sugar (P=0.024) and body weight (P=0.005) showed significant decreases in the experimental group in the post-test, compared to the pre-test. Furthermore, a significant correlation was observed between the changes in Isthmin-1 levels after eight weeks of HIIT and the changes in insulin levels (P=0.014).
Conclusions: It appears that eight weeks of HIIT modulates insulin resistance by increasing Isthmin-1 levels in obese diabetic rats, in addition to improving body composition.
Type of Study: Orginal Research |
Subject:
Basic Sciences
Received: 2025/09/5 | Accepted: 2025/10/12 | Published: 2026/03/29
Received: 2025/09/5 | Accepted: 2025/10/12 | Published: 2026/03/29
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