Journal of North Khorasan

Abstract Background & Objectives: Plant extracts are rich sources of natural antibacterial compounds. In recent decades, the need for using natural preservative in foods, has promoted extensive scientific research on plants extracts and essential oils. The purpose of this investigation was to determine the antibacterial activities of root extracts of Biebersteinia multifida DC. Material and Methods: the hexane, chloroform and ethanol roots extracts of Biebersteinia multifida DC. are obtained by maceration method. Antibacterial activity of extracts determined against Staphylococcus aureus (PTCC 1431), Salmonella enterica (PTCC 1709), Escherichia coli (PTCC 1399), Bacillus cereus (PTCC 1015), Enterobacter aerogenes (PTCC 1229), Clostridium perfringens (PTCC 1765). The micro-dilution method was applied for the determination of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of selected bacteria strains. Results: gram positive bacteria were more sensitive to the extracts to gram negative bacteria. The minimum inhibitory concentration and the minimum bactericidal concentration against C. perfringens were 0.195 mg mL-1 and 0.391 mg mL-1. Conclusion: The findings indicate that hexane and ethanol roots extracts of Biebersteinia multifida DC. exerts antibacterial effects on C. perfringens. More studies, such as examinations in food models are needed to unravel the antimicrobial effects of this plant extracts.